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Table II.

Dosage Effect of Txk Transgene in H-Y TCR Transgenic Female Mice

No. DP T3.70high thymocytesNo. CD8 SP T3.70high thymocytes
  ×106 ×106 
Experiment 1 txk tg 7.56 4.71 
txk tg/tg 8.58 2.20 
Experiment 2 txk tg 4.69 5.53 
txk tg/tg 6.04 3.83 
No. DP T3.70high thymocytesNo. CD8 SP T3.70high thymocytes
  ×106 ×106 
Experiment 1 txk tg 7.56 4.71 
txk tg/tg 8.58 2.20 
Experiment 2 txk tg 4.69 5.53 
txk tg/tg 6.04 3.83 

Thymocytes from littermate H-Y × txk hemizygous transgenic female mice (txk tg) and H-Y × txk homozygous female mice (txk tg/tg) were stained with anti-CD4, anti-CD8, and T3.70 antibodies and analyzed by flow cytometry. Numbers of T3.70high DP and T3.70high CD8 SP thymocytes were determined.

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