Dosage Effect of Txk Transgene in H-Y TCR Transgenic Female Mice
| . | . | No. DP T3.70high thymocytes . | No. CD8 SP T3.70high thymocytes . |
|---|---|---|---|
| ×106 | ×106 | ||
| Experiment 1 | txk tg | 7.56 | 4.71 |
| txk tg/tg | 8.58 | 2.20 | |
| Experiment 2 | txk tg | 4.69 | 5.53 |
| txk tg/tg | 6.04 | 3.83 |
| . | . | No. DP T3.70high thymocytes . | No. CD8 SP T3.70high thymocytes . |
|---|---|---|---|
| ×106 | ×106 | ||
| Experiment 1 | txk tg | 7.56 | 4.71 |
| txk tg/tg | 8.58 | 2.20 | |
| Experiment 2 | txk tg | 4.69 | 5.53 |
| txk tg/tg | 6.04 | 3.83 |
Thymocytes from littermate H-Y × txk hemizygous transgenic female mice (txk tg) and H-Y × txk homozygous female mice (txk tg/tg) were stained with anti-CD4, anti-CD8, and T3.70 antibodies and analyzed by flow cytometry. Numbers of T3.70high DP and T3.70high CD8 SP thymocytes were determined.