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Table 1

Increased Cell Cycling with CD3/FasL Costimulation

StimulusPercentage of cells in S+G2+M
Day 1Day 2Day 3Day 4Day 5
Experiment 1      
Medium 2.6 2.6 3.6 3.4 3.9 
α-CD3 6.8 5.0 8.8 5.8 7.5 
α-CD3/FasL 11.9 9.2 14.7 9.5 10.1 
FasL 3.4 4.5 4.2 5.2 4.6 
α-CD3/α-CD28 6.2 10.5 10.3 9.7 7.9 
Experiment 2      
Medium   5.1 4.6 2.1 
α-CD3   6.5 7.5 4.2 
α-CD3/FasL   9.6 15.2 12.2 
FasL   3.6 3.5 1.7 
StimulusPercentage of cells in S+G2+M
Day 1Day 2Day 3Day 4Day 5
Experiment 1      
Medium 2.6 2.6 3.6 3.4 3.9 
α-CD3 6.8 5.0 8.8 5.8 7.5 
α-CD3/FasL 11.9 9.2 14.7 9.5 10.1 
FasL 3.4 4.5 4.2 5.2 4.6 
α-CD3/α-CD28 6.2 10.5 10.3 9.7 7.9 
Experiment 2      
Medium   5.1 4.6 2.1 
α-CD3   6.5 7.5 4.2 
α-CD3/FasL   9.6 15.2 12.2 
FasL   3.6 3.5 1.7 

T cells were activated by the indicated stimulus, and samples were taken on the days shown, permeabilized, and stained with PI. Cell cycle analysis was performed by flow cytometry, and gates were set for the S+G2+M phases of the cell cycle.

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