Development of Disease Correlates with a Significant Increase in the Total Number of Leukocytes and CD4+ Cells Recovered from the LP
| Phenotype of CD4+ T cells used for reconstitution . | mAb treatment . | Total no. of LP leukocytes . | Total no. of CD4+ cells . |
|---|---|---|---|
| ×103 ± SEM | ×103 ± SEM | ||
| 4 × 105 WT CD45RBhigh | – | 136.0 ± 34.9 | 92.7 ± 10.2 |
| 2 × 105 IL-10−/− CD45RBlow | – | 64.4 ± 29.7 | 43.9 ± 22.8 |
| 4 × 105 WT CD45RBhigh | |||
| + 2 × 105 WT CD45RBlow | anti–IL-10R | 176.3 ± 104.5 | 133.3 ± 88.6 |
| 4 × 105 WT CD45RBhigh | Isotype | ||
| + 2 × 105 CD45RBlow | control | 7.9 ± 0.9 | 5.4 ± 0.5 |
| Phenotype of CD4+ T cells used for reconstitution . | mAb treatment . | Total no. of LP leukocytes . | Total no. of CD4+ cells . |
|---|---|---|---|
| ×103 ± SEM | ×103 ± SEM | ||
| 4 × 105 WT CD45RBhigh | – | 136.0 ± 34.9 | 92.7 ± 10.2 |
| 2 × 105 IL-10−/− CD45RBlow | – | 64.4 ± 29.7 | 43.9 ± 22.8 |
| 4 × 105 WT CD45RBhigh | |||
| + 2 × 105 WT CD45RBlow | anti–IL-10R | 176.3 ± 104.5 | 133.3 ± 88.6 |
| 4 × 105 WT CD45RBhigh | Isotype | ||
| + 2 × 105 CD45RBlow | control | 7.9 ± 0.9 | 5.4 ± 0.5 |
RAG-2−/− or C.B-17 SCID recipients were reconstituted with cell subsets as indicated. 8–12 wk after cell reconstitution, cells from LP were isolated. Data represent the number ± SEM of two to five animals per group from two independent experiments.