IL-12 p70 and p40 Production by Isolated Human Thymic DCs in the Presence or Absence of IL-4
| Experiment . | . | . | . |
|---|---|---|---|
| 1 | p70 (pg/ml) | 260 | 542 |
| p40 (pg/ml) | 12,992 | 5,440 | |
| 2 | p70 (pg/ml) | 6 | 76 |
| p40 (pg/ml) | 906 | 391 | |
| IL-4 | − | + |
| Experiment . | . | . | . |
|---|---|---|---|
| 1 | p70 (pg/ml) | 260 | 542 |
| p40 (pg/ml) | 12,992 | 5,440 | |
| 2 | p70 (pg/ml) | 6 | 76 |
| p40 (pg/ml) | 906 | 391 | |
| IL-4 | − | + |
Human thymic DCs (9 × 104 DCs per 200 μl in experiment 1, or 5 × 104 DCs per 200 μl in experiment 2) were incubated with CD40L in the presence of GM-CSF and IFN-γ with or without IL-4 for 3 d (experiment 1) or 2 d (experiment 2). Supernatants were assayed for IL-12 p70 and p40 by ELISA.