Table 1

Oligonucleotides and Restriction Enzymes for Chimera Construction

ChimeraOligonucleotidesRestriction enzymes
A55/B Sense: 5′-gggaagcttatggaaaccaacttct-3′ HindIII, Eco47III 
 Antisense: 5′-aacagcgcttcatccggaatccagc-3′  
B/A62–262/B Sense: 5′-gttagcgctcagtcaccaccatct-3′ Eco47III, EcoNI 
 Antisense: 5′-aacctcagccagggcaaccagttgaa-3′  
B/A254 Sense: 5′-gaacctggctgaggccgtctggctcaag-3′ EcoNI, XhoI 
 Antisense: 5′-cttctcgagtcacattgcctgtaac-3′  
B/A299 Sense: 5′-gtgcgccggcggctttgtgggccaagactt-3′ SgrAI, XhoI 
 Antisense: 5′-cttctcgagtcacattgcctgtaac-3′  
ChimeraOligonucleotidesRestriction enzymes
A55/B Sense: 5′-gggaagcttatggaaaccaacttct-3′ HindIII, Eco47III 
 Antisense: 5′-aacagcgcttcatccggaatccagc-3′  
B/A62–262/B Sense: 5′-gttagcgctcagtcaccaccatct-3′ Eco47III, EcoNI 
 Antisense: 5′-aacctcagccagggcaaccagttgaa-3′  
B/A254 Sense: 5′-gaacctggctgaggccgtctggctcaag-3′ EcoNI, XhoI 
 Antisense: 5′-cttctcgagtcacattgcctgtaac-3′  
B/A299 Sense: 5′-gtgcgccggcggctttgtgggccaagactt-3′ SgrAI, XhoI 
 Antisense: 5′-cttctcgagtcacattgcctgtaac-3′  

See Materials and Methods for details.

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