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Table 2.

Ig secretion by IL-21–stimulated normal, STAT3MUT, and STAT1MUT naive and memory B cells

Cell type and culture Ig secretion 
 IgM IgG IgA 
 Normal STAT3MUT STAT1MUT Normal STAT3MUT STAT1MUT Normal STAT3MUT STAT1MUT 
 ng/ml ng/ml ng/ml ng/ml ng/ml ng/ml ng/ml ng/ml ng/ml 
Naive B cells          
CD40L 2.0 ± 1.0 5.0 ± 2.4a <0. 1a <1 <1a <1a <1 <1a <1a 
+IL-21 14,640 ± 2,021 444 ± 177d 10,801 ± 3,183a 267 ± 80 7.3 ± 2.7b 86 ± 40a 365 ± 106 4.6 ± 2.6b 174 ± 40a 
Memory B cells          
CD40L 166 ± 51 100 ± 25a 2.5 ± 2.5a 6.1 ± 1.4 11.8 ± 4.7a 0.2 ± 0.14a 21.3 ± 6.1 26.2 ± 10.6a <1a 
+IL-21 29,389 ± 4,107 15,214 ± 1,905b 7,396 ± 3,097d 4,202 ± 1,122 5,651 ± 1,743a 953 ± 195a 25,208 ± 4,134 13,330 ± 4,027a 4,265 ± 989c 
Cell type and culture Ig secretion 
 IgM IgG IgA 
 Normal STAT3MUT STAT1MUT Normal STAT3MUT STAT1MUT Normal STAT3MUT STAT1MUT 
 ng/ml ng/ml ng/ml ng/ml ng/ml ng/ml ng/ml ng/ml ng/ml 
Naive B cells          
CD40L 2.0 ± 1.0 5.0 ± 2.4a <0. 1a <1 <1a <1a <1 <1a <1a 
+IL-21 14,640 ± 2,021 444 ± 177d 10,801 ± 3,183a 267 ± 80 7.3 ± 2.7b 86 ± 40a 365 ± 106 4.6 ± 2.6b 174 ± 40a 
Memory B cells          
CD40L 166 ± 51 100 ± 25a 2.5 ± 2.5a 6.1 ± 1.4 11.8 ± 4.7a 0.2 ± 0.14a 21.3 ± 6.1 26.2 ± 10.6a <1a 
+IL-21 29,389 ± 4,107 15,214 ± 1,905b 7,396 ± 3,097d 4,202 ± 1,122 5,651 ± 1,743a 953 ± 195a 25,208 ± 4,134 13,330 ± 4,027a 4,265 ± 989c 

Naive and memory B cells were sort-purified from normal healthy donors (n = 17), STAT3MUT patients (n = 8), or STAT1MUT patients (n = 6) and then cultured with CD40L alone or together with IL-21. The levels of secreted IgM, IgG, and IgA were determined by ELISA after 10–12 d. The values represent the mean ± SEM from the indicated number of donors/patients and correspond to the data depicted in Fig. 3 (A and B). Statistical analyses were performed using one-way ANOVA; differences are indicated for normal donors compared with STAT3MUT or STAT1MUT B cells.

a

Not significant.

b

P < 0.01.

c

P < 0.001.

d

P < 0.0001.

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