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Table 2

ELISPOT Analysis of Virus-specific T Cells in the BAL, Lung Tissue, MLNs, and Spleens of C57BL/6 Mice After Resolution of a Primary Sendai Virus Infection

Sendai HN421–436Sendai NP324–332
Sen-HN421–436 specific among CD4+Total no. of Sen-HN421–436–specific cells per mouseSen-NP324–332 specific among CD8+Total no. of Sen-NP324–332–specific cells per mouse
   
BAL (n = 9) 13.2 1,964 59.5 11,032 
Lung tissue (n = 8) 2.1 1,679 13.9 7,400 
MLNs (n = 8) 0.11 1,512 0.37 4,992 
Spleen (n = 3) 0.06 18,320 0.44 88,578 
Sendai HN421–436Sendai NP324–332
Sen-HN421–436 specific among CD4+Total no. of Sen-HN421–436–specific cells per mouseSen-NP324–332 specific among CD8+Total no. of Sen-NP324–332–specific cells per mouse
   
BAL (n = 9) 13.2 1,964 59.5 11,032 
Lung tissue (n = 8) 2.1 1,679 13.9 7,400 
MLNs (n = 8) 0.11 1,512 0.37 4,992 
Spleen (n = 3) 0.06 18,320 0.44 88,578 

Mice were intranasally infected with 500 EID50 Sendai virus and tissues were analyzed on day 41 after infection. Data are representative of two independent experiments.

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