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Table I.

Lymphocytes Counts in TACI:FC Transgenic Mice and Control Littermates



Cell number (×106)





Controls
TACI:Fc
P value
Controls
TACI:Fc
P value
Spleen       
All cells 63.5 ± 7.1 25.0 ± 2.9 ** 100 100  
T cells 12.3 ± 1.2 9.3 ± 1.4  19.5 ± 2.1 36.9 ± 1.8 ** 
CD4+ 9.1 ± 0.8 6.2 ± 0.6 14.4 ± 1.3 25.0 ± 1.1 ** 
CD8+ 3.3 ± 0.3 2.9 ± 0.9  5.3 ± 0.7 11.5 ± 2.1 
B cells 44.8 ± 5.0 9.3 ± 3.7 ** 70.5 ± 2.2 37.8 ± 5.1 ** 
T1 1.9 ± 0.3 3.3 ± 0.4  3.0 ± 0.3 13.5 ± 2.6  
T2 2.6 ± 0.1 0.045 ± 0.022 *** 4.2 ± 0.3 0.19 ± 0.09 *** 
MZ 1.4 ± 0.3 0.050 ± 0.014 ** 2.3 ± 0.6 0.21 ± 0.08 ** 
FO 36.1 ± 5.6 2.0 ± 0.3 ** 56.6 ± 2.6 8.3 ± 1.7 ** 
Thymus       
All cells 122.3 ± 12.0 133.7 ± 14.4  100 100  
CD4+/CD8+ 107.2 ±10.5 113.5 ± 12.9  87.6 ± 0.3 85.0 ± 3.4  
CD4+ 0.84 ± 0.05 1.17 ± 0.33  6.9 ± 0.2 8.7 ± 2.2  
CD8+ 0.17 ± 0.04 0.24 ± 0.08  1.4 ± 0.4 1.8 ± 0.6  
Immat. CD8+ 0.049 ± 0.023 0.47 ± 0.005  0.41 ± 0.19 0.36 ± 0.08  
γδ 0.016 ± 0.003 0.021 ± 0.004  0.13 ± 0.01 0.16 ± 0.02  
Bone marrow       
All cells 44.8 ± 3.1 32.1 ± 3.0 100 100  
Pro-B/pre-B 4.6 ± 0.4 4.2 ± 1.4  10.2 ± 0.3 13.1 ± 3.3 
Pre-B/Imm. B 13.6 ± 0.5 8.8 ± 0.8 30.6 ± 1.5 27.5 ± 1.8  
Recirculating B 3.0 ± 0.2 0.53 ± 0.09 *** 6.6 ± 0.5 1.6 ± 0.1 *** 
Lymph node       
CD4+ ND ND  36.1 ± 2.4 48.6 ± 1.0 
CD8+ ND ND  26.4 ± 2.3 33.5 ± 1.8 
B cells ND ND  35.5 ± 2.0 5.6 ± 2.2 ** 
PBLs       
All cells 8.2 ± 1.1 5.5 ± 0.8 ** 100 100  
 2.5 ± 0.3 2.9 ± 0.5  30.5 ± 1.8 51.7 ± 3.8 ** 
 1.5 ± 0.2 1.8 ± 0.3 18.6 ± 1.3 31.8 ± 2.2 ** 
 0.98 ± 0.15 1.1 ± 0.2  12.0 ± 0.6 19.2 ± 1.6 ** 
 4.8 ± 0.7 1.4 ± 0.1 ** 58.1 ± 2.9 26.5 ± 5.6 ** 
 0.28 ± 0.08 0.47 ± 0.07 ** 3.3 ± 0.7 8.7 ± 1.2 ** 
 3.4 ± 0.6 0.35 ± 0.04 ** 41.0 ± 2.6 6.5 ± 0.5 *** 
PEL's    Ratio
 
  
B2/B1 ratio    0.48 ± 0.16
 
0.056 ± 0.039
 
** 


Cell number (×106)





Controls
TACI:Fc
P value
Controls
TACI:Fc
P value
Spleen       
All cells 63.5 ± 7.1 25.0 ± 2.9 ** 100 100  
T cells 12.3 ± 1.2 9.3 ± 1.4  19.5 ± 2.1 36.9 ± 1.8 ** 
CD4+ 9.1 ± 0.8 6.2 ± 0.6 14.4 ± 1.3 25.0 ± 1.1 ** 
CD8+ 3.3 ± 0.3 2.9 ± 0.9  5.3 ± 0.7 11.5 ± 2.1 
B cells 44.8 ± 5.0 9.3 ± 3.7 ** 70.5 ± 2.2 37.8 ± 5.1 ** 
T1 1.9 ± 0.3 3.3 ± 0.4  3.0 ± 0.3 13.5 ± 2.6  
T2 2.6 ± 0.1 0.045 ± 0.022 *** 4.2 ± 0.3 0.19 ± 0.09 *** 
MZ 1.4 ± 0.3 0.050 ± 0.014 ** 2.3 ± 0.6 0.21 ± 0.08 ** 
FO 36.1 ± 5.6 2.0 ± 0.3 ** 56.6 ± 2.6 8.3 ± 1.7 ** 
Thymus       
All cells 122.3 ± 12.0 133.7 ± 14.4  100 100  
CD4+/CD8+ 107.2 ±10.5 113.5 ± 12.9  87.6 ± 0.3 85.0 ± 3.4  
CD4+ 0.84 ± 0.05 1.17 ± 0.33  6.9 ± 0.2 8.7 ± 2.2  
CD8+ 0.17 ± 0.04 0.24 ± 0.08  1.4 ± 0.4 1.8 ± 0.6  
Immat. CD8+ 0.049 ± 0.023 0.47 ± 0.005  0.41 ± 0.19 0.36 ± 0.08  
γδ 0.016 ± 0.003 0.021 ± 0.004  0.13 ± 0.01 0.16 ± 0.02  
Bone marrow       
All cells 44.8 ± 3.1 32.1 ± 3.0 100 100  
Pro-B/pre-B 4.6 ± 0.4 4.2 ± 1.4  10.2 ± 0.3 13.1 ± 3.3 
Pre-B/Imm. B 13.6 ± 0.5 8.8 ± 0.8 30.6 ± 1.5 27.5 ± 1.8  
Recirculating B 3.0 ± 0.2 0.53 ± 0.09 *** 6.6 ± 0.5 1.6 ± 0.1 *** 
Lymph node       
CD4+ ND ND  36.1 ± 2.4 48.6 ± 1.0 
CD8+ ND ND  26.4 ± 2.3 33.5 ± 1.8 
B cells ND ND  35.5 ± 2.0 5.6 ± 2.2 ** 
PBLs       
All cells 8.2 ± 1.1 5.5 ± 0.8 ** 100 100  
 2.5 ± 0.3 2.9 ± 0.5  30.5 ± 1.8 51.7 ± 3.8 ** 
 1.5 ± 0.2 1.8 ± 0.3 18.6 ± 1.3 31.8 ± 2.2 ** 
 0.98 ± 0.15 1.1 ± 0.2  12.0 ± 0.6 19.2 ± 1.6 ** 
 4.8 ± 0.7 1.4 ± 0.1 ** 58.1 ± 2.9 26.5 ± 5.6 ** 
 0.28 ± 0.08 0.47 ± 0.07 ** 3.3 ± 0.7 8.7 ± 1.2 ** 
 3.4 ± 0.6 0.35 ± 0.04 ** 41.0 ± 2.6 6.5 ± 0.5 *** 
PEL's    Ratio
 
  
B2/B1 ratio    0.48 ± 0.16
 
0.056 ± 0.039
 
** 

Cell numbers are in million per organ. For bone marrow, numbers are for one tibia and one femur. For PBLs, numbers are for 1 ml of blood. Three mice (9-wk-old females) were analyzed in each group.

P values: *0.2 ≥ P ≥ 0.05; **0.05 ≥ P ≥ 0.01; ***P < 0.01.

Population definition: Spleen: T1 (B220+, CD23, CD21, IgM+); T2 (B220+, CD23+, CD21++, IgM++); MZ (B220+, CD23, CD21++, IgM++); FO (B220+, CD23+, CD21+, IgM+). Thymus: Immature CD4+ (CD4+, TCR-αβ2); γδ (CD4, CD8, TCR-γδ1). Bone marrow: pro-B/pre-B (B220+, CD43+); pre-B/immature B (B220+, CD43, IgD− or low); recirculating B (B220+, CD43, IgD+). PBLs: immature B (B220+, CD23); mature B (B220+, CD23+). PELs: B2 (B220+, CD23+, CD5); B1 (220+, IgM+, CD23, CD5− or +).

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