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Table I.

Cell Surface Markers Expressed by the NK T Cell Population in B10.Pl Mice



Spleen

Liver
Markers
PBS
α-GalCer
PBS
α-GalCer
MCD1d-tetramer 2.56 1.78 18.67 0.1 
MCD1d-tetramer plus TCR 1.78 1.41 22.65 1.54 
NK1.1 3.37 3.22 41.62 36.96 
NK1.1 plus TCR 1.39 0.8 21.83 0.92 
NK1.1 plus Vβ8.1/2 0.11 0.05 10.64 1.77 
NK1.1 plus Vβ7 0.8 0.53 4.13 0.38 
NK1.1 plus Vβ2 0.05 0.11 2.36 n/d 
Unloaded tetramer
 
1.02
 
1.08
 
0.65
 
0.44
 


Spleen

Liver
Markers
PBS
α-GalCer
PBS
α-GalCer
MCD1d-tetramer 2.56 1.78 18.67 0.1 
MCD1d-tetramer plus TCR 1.78 1.41 22.65 1.54 
NK1.1 3.37 3.22 41.62 36.96 
NK1.1 plus TCR 1.39 0.8 21.83 0.92 
NK1.1 plus Vβ8.1/2 0.11 0.05 10.64 1.77 
NK1.1 plus Vβ7 0.8 0.53 4.13 0.38 
NK1.1 plus Vβ2 0.05 0.11 2.36 n/d 
Unloaded tetramer
 
1.02
 
1.08
 
0.65
 
0.44
 

Monocytes from the liver and spleens of each PBS or α-GalCer immunized mouse were stained and analyzed by flow cytometry as described in Materials and Methods. These data are representative of two independent experiments.

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