Sporozoite Distributions in Anopheles stephensi Mosquitoes Infected with Wild-type, maebl(−), and trap(−) Populations
Parasite population . | Percent of infected guts . | . | No. of sporozoites/mosquito . | . | |
|---|---|---|---|---|---|
. | . | Midgut . | Hemolymph . | Salivary gland . | |
| WT | ND | 66,200 ± 12,300 | 2,867 ± 809 | 13,733 ± 3,130 | |
| maebl(+) 1 | 85.7 ± 2.3 | 79,900 ± 8,684 | 5,633 ± 285 | 11,933 ± 1,430 | |
| maebl(+) 2 | 85.3 ± 3.4 | 69,233 ± 17,120 | 4,000 ± 416 | 12,233 ± 649 | |
| maebl(−) 1 | ND | 136,075 ± 19,900 | 12,575 ± 1,738 | 2.3 ± 0.4 | |
| maebl(−) 2 | ND | 60,500 ± 12,040 | 9,667 ± 3,517 | 1.4 ± 0.9 | |
| maebl(−) 3 | 96.8 ± 1.4 | 127,400 ± 19,400 | 15,267 ± 3,019 | 1.4 ± 0.3 | |
| maebl(−) 4 | 97.0 ± 3.0 | 82,233 ± 15,070 | 15,333 ± 1,868 | 2.8 ± 0.5 | |
| trap(−) 1 | 94.7 ± 2.9 | 111,167 ± 10,900 | 9,700 ± 700 | 32.4 ± 4.6 | |
| trap(−) 2 | 98.0 ± 1.0 | 115,600 ± 3,110 | 7,867 ± 498 | 49.4 ± 13.3 | |
Parasite population . | Percent of infected guts . | . | No. of sporozoites/mosquito . | . | |
|---|---|---|---|---|---|
. | . | Midgut . | Hemolymph . | Salivary gland . | |
| WT | ND | 66,200 ± 12,300 | 2,867 ± 809 | 13,733 ± 3,130 | |
| maebl(+) 1 | 85.7 ± 2.3 | 79,900 ± 8,684 | 5,633 ± 285 | 11,933 ± 1,430 | |
| maebl(+) 2 | 85.3 ± 3.4 | 69,233 ± 17,120 | 4,000 ± 416 | 12,233 ± 649 | |
| maebl(−) 1 | ND | 136,075 ± 19,900 | 12,575 ± 1,738 | 2.3 ± 0.4 | |
| maebl(−) 2 | ND | 60,500 ± 12,040 | 9,667 ± 3,517 | 1.4 ± 0.9 | |
| maebl(−) 3 | 96.8 ± 1.4 | 127,400 ± 19,400 | 15,267 ± 3,019 | 1.4 ± 0.3 | |
| maebl(−) 4 | 97.0 ± 3.0 | 82,233 ± 15,070 | 15,333 ± 1,868 | 2.8 ± 0.5 | |
| trap(−) 1 | 94.7 ± 2.9 | 111,167 ± 10,900 | 9,700 ± 700 | 32.4 ± 4.6 | |
| trap(−) 2 | 98.0 ± 1.0 | 115,600 ± 3,110 | 7,867 ± 498 | 49.4 ± 13.3 | |
Mosquitoes fed on mice infected with wild-type polyclonal populations (WT) or respective parasite populations. 20–24 d after blood feeding, sporozoites were separately collected from the salivary glands, the hemolymph, and the midgut as previously described (15). Each value is the mean with its standard error of at least three independent experiments using 30–40 mosquitoes in each experiment.