Infectivities to Rats and Proportions of Gliding Motility–positive Parasites in Wild-type, maebl(−), and trap(−) Populations
Parasite population . | Percent gliding motilitya . | No. of injected midgut sporozoites . | No. of infected ratsb . | Prep. periodc . | No. of injected midgut sporozoites . | No. of infected ratsb . | Prep. periodc . |
|---|---|---|---|---|---|---|---|
| d | d | ||||||
| WT | 6.1 | 50,000 | 9/15 | 5.2 | 100,000 | 12/15 | 5.2 |
| maebl(+) 1 | 7.8 | 7/10 | 4.6 | 8/10 | 4.6 | ||
| maebl(+) 2 | 7.6 | 5/10 | 5.6 | 9/10 | 4.7 | ||
| Average | 5.1 | 4.8 | |||||
| maebl(−) 1 | 5.9 | 50,000 | 10/10 | 5.1 | 100,000 | 9/10 | 4.7 |
| maebl(−) 2 | 7.5 | 8/15 | 5.0 | 14/15 | 4.4 | ||
| maebl(−) 3 | 7.2 | 4/10 | 5.2 | 10/10 | 4.6 | ||
| maebl(−) 4 | 7.1 | 9/10 | 4.7 | 10/10 | 4.7 | ||
| Average | 5.0 | 4.6 | |||||
| trap(−) 1 | 0.0 | 500,000 | 0/4 | — | |||
| trap(−) 2 | 0.0 | 0/4 | — |
Parasite population . | Percent gliding motilitya . | No. of injected midgut sporozoites . | No. of infected ratsb . | Prep. periodc . | No. of injected midgut sporozoites . | No. of infected ratsb . | Prep. periodc . |
|---|---|---|---|---|---|---|---|
| d | d | ||||||
| WT | 6.1 | 50,000 | 9/15 | 5.2 | 100,000 | 12/15 | 5.2 |
| maebl(+) 1 | 7.8 | 7/10 | 4.6 | 8/10 | 4.6 | ||
| maebl(+) 2 | 7.6 | 5/10 | 5.6 | 9/10 | 4.7 | ||
| Average | 5.1 | 4.8 | |||||
| maebl(−) 1 | 5.9 | 50,000 | 10/10 | 5.1 | 100,000 | 9/10 | 4.7 |
| maebl(−) 2 | 7.5 | 8/15 | 5.0 | 14/15 | 4.4 | ||
| maebl(−) 3 | 7.2 | 4/10 | 5.2 | 10/10 | 4.6 | ||
| maebl(−) 4 | 7.1 | 9/10 | 4.7 | 10/10 | 4.7 | ||
| Average | 5.0 | 4.6 | |||||
| trap(−) 1 | 0.0 | 500,000 | 0/4 | — | |||
| trap(−) 2 | 0.0 | 0/4 | — |
Only sporozoites displaying typical circular gliding were counted.
Number of infected rats/number of rats injected with midgut sporozoite suspension.
Number of days between sporozoite injection and detection of at least one erythrocytic stage upon a 10-min examination of a Giemsa-stained blood smear. The value is the mean value of successful infection.
Sporozoites were collected from mosquitoes infected with respective populations 20–24 d after an infective blood meal. For analysis of gliding motility, the hemolymph sporozoites were kept for 2 h in 3% BSA at 4°C and then gliding on an uncoated microscope was observed under a phase-contrast microscope. For analysis of infectivity, midgut sporozoites were injected into 3-wk-old Wistar rats.