Rockefeller University Press

Table II.

Repopulating Ability of Hematopoietic Progenitors in Irradiated Hosts Is Enhanced by the Absence of Lnk

		Percent chimerism (Ly5.2⁺ cells, %)
Experiment	Tested donor	Bone marrowB220^loCD43⁻	Bone marrowB220⁻CD43⁺	ThymusCD4⁺CD8⁺
Exp. 1	Ly5.1 alone	2.3	7.4	0.1
	lnk^+/+	10.9	28.9	0.1
	lnk^−/−	87.1	48.9	99.8
	lnk^−/−	85.1	78.1	87.5
Exp. 2	lnk^+/+	16.6	23.9	17.2
	lnk^+/+	18.0	16.9	16.1
	lnk^−/−	97.5	93.9	99.2
	lnk^−/−	97.6	95.8	99.4
Exp. 3	Ly5.1 alone	5.4	9.9	2.2
	lnk^+/+	33.3	42.3	30.4
	lnk^−/−	94.8	96.3	99.7
	lnk^−/−	95.3	95.2	98.2

		Percent chimerism (Ly5.2⁺ cells, %)
Experiment	Tested donor	Bone marrowB220^loCD43⁻	Bone marrowB220⁻CD43⁺	ThymusCD4⁺CD8⁺
Exp. 1	Ly5.1 alone	2.3	7.4	0.1
	lnk^+/+	10.9	28.9	0.1
	lnk^−/−	87.1	48.9	99.8
	lnk^−/−	85.1	78.1	87.5
Exp. 2	lnk^+/+	16.6	23.9	17.2
	lnk^+/+	18.0	16.9	16.1
	lnk^−/−	97.5	93.9	99.2
	lnk^−/−	97.6	95.8	99.4
Exp. 3	Ly5.1 alone	5.4	9.9	2.2
	lnk^+/+	33.3	42.3	30.4
	lnk^−/−	94.8	96.3	99.7
	lnk^−/−	95.3	95.2	98.2

Lin⁻ bone marrow cells obtained from lnk^+/+ or lnk^−/− mice (Ly 5.2) were transferred into lethally irradiated (9.5 Gy) recipient mice together with a fivefold excess of Lin⁻ cells prepared from normal B6-Ly5.1 mice. Bone marrow cells and thymocytes were isolated 10–12 wk after transfer, and percentage of Ly5.2⁺ cells in the indicated cell fractions were determined.

View Large

This Feature Is Available To Subscribers Only