Comparable Repopulating Ability of HSCs Obtained from lnk−/−Kit+/+ or lnk−/−KitW/+ Mice
. | . | . | Percent chimerism (Ly5.21 cells, %) . | . | . | ||
|---|---|---|---|---|---|---|---|
| Experiment . | Tested donor . | Testee:Competitor . | Bone marrowB220loCD43− . | Bone marrowB220loCD43+ . | ThymusCD4+CD8+ . | ||
| Exp. 1 | Ly5.1 alone | 4.7 | 5.1 | 0.3 | |||
| lnk−/−Kit+/+ | 1:10 | 98.1 | 76.6 | 94.8 | |||
| lnk−/−KitW/+ | 1:10 | 98.4 | 73.1 | 77.5 | |||
| lnk−/−Kit+/+ | 1:20 | 98.5 | 71.1 | 96.1 | |||
| lnk−/−KitW/+ | 1:20 | 94.4 | 64.4 | 24.6 | |||
| Exp. 2 | Ly5.1 alone | 4.4 | 3.1 | 0.1 | |||
| lnk−/−Kit+/+ | 1:10 | 81.2 | 65.8 | 81.2 | |||
| lnk−/−KitW/+ | 1:10 | 87.6 | 77.1 | 76.9 | |||
| lnk−/−Kit+/+ | 1:20 | 86.1 | 67.3 | 97.0 | |||
| lnk−/−KitW/+ | 1:20 | 87.2 | 74.5 | 86.8 | |||
. | . | . | Percent chimerism (Ly5.21 cells, %) . | . | . | ||
|---|---|---|---|---|---|---|---|
| Experiment . | Tested donor . | Testee:Competitor . | Bone marrowB220loCD43− . | Bone marrowB220loCD43+ . | ThymusCD4+CD8+ . | ||
| Exp. 1 | Ly5.1 alone | 4.7 | 5.1 | 0.3 | |||
| lnk−/−Kit+/+ | 1:10 | 98.1 | 76.6 | 94.8 | |||
| lnk−/−KitW/+ | 1:10 | 98.4 | 73.1 | 77.5 | |||
| lnk−/−Kit+/+ | 1:20 | 98.5 | 71.1 | 96.1 | |||
| lnk−/−KitW/+ | 1:20 | 94.4 | 64.4 | 24.6 | |||
| Exp. 2 | Ly5.1 alone | 4.4 | 3.1 | 0.1 | |||
| lnk−/−Kit+/+ | 1:10 | 81.2 | 65.8 | 81.2 | |||
| lnk−/−KitW/+ | 1:10 | 87.6 | 77.1 | 76.9 | |||
| lnk−/−Kit+/+ | 1:20 | 86.1 | 67.3 | 97.0 | |||
| lnk−/−KitW/+ | 1:20 | 87.2 | 74.5 | 86.8 | |||
Bone marrow cells obtained from lnk−/− Kit+/+ or lnk−/−KitW/+ mice (Ly5.2) were transferred into lethally irradiated (9.5 Gy) recipient mice together with 10-fold or 20-fold excess of competitor bone marrow cells prepared from normal B6-Ly5.1 mice. Bone marrow cells and thymocytes were isolated 10–12 wk after transfer, and percentage of Ly5.2+ cells in the indicated cell fractions was determined.