Genotype Analysis of the Progeny from Hzf Heterozygous Intercrosses
. | Number of mice of each genotype . | . | . | . | Percentage . | ||
|---|---|---|---|---|---|---|---|
| Stage . | +/+ . | +/− . | −/− . | Total . | −/− . | ||
| % | |||||||
| 1 wk | 10 | 22 | 12 | 44 | 27 | ||
| 2 wk | 6 | 22 | 8 | 36 | 22 | ||
| 3 wk | 32 | 90 | 24 | 146 | 16 | ||
| 4 wk | 16 | 27 | 10 | 53 | 19 | ||
| Total | 295 | ||||||
. | Number of mice of each genotype . | . | . | . | Percentage . | ||
|---|---|---|---|---|---|---|---|
| Stage . | +/+ . | +/− . | −/− . | Total . | −/− . | ||
| % | |||||||
| 1 wk | 10 | 22 | 12 | 44 | 27 | ||
| 2 wk | 6 | 22 | 8 | 36 | 22 | ||
| 3 wk | 32 | 90 | 24 | 146 | 16 | ||
| 4 wk | 16 | 27 | 10 | 53 | 19 | ||
| Total | 295 | ||||||
Breeding pairs were set up between Hzf +/− males and females (129 × CD1 mixed background). Tails of newborn and weaning pups were collected at the time of 1, 2, 3, and 4 wk after birth. The genotypes were determined by PCR analyses, as described in Materials and Methods. Each genotyping was confirmed by Southern blot analyses.