Anti-CD4 mAb Treatment Does Not Induce LLO91–99–specific CD8+ Memory T Cell Proliferation in the Absence of Antigen Stimulation
. | Percent LLO91–99-tetramer+ of CD8+ cells . | LLO91–99-tetramer+CD8+ cells/spleen . |
|---|---|---|
| Naive | <0.02 | <1,000 |
| + anti-CD4 mAb | <0.02 | <1,000 |
| L. monocytogenes | 0.06 ± 0.01 | 1,700 ± 100 |
| + anti-CD4 mAb | 0.07 ± 0.04 | 2,700 ± 1,600 |
| Gene gun | 0.09 ± 0.04 | 3,900 ± 2,800 |
| + anti-CD4 mAb | 0.10 ± 0.03 | 3,400 ± 900 |
. | Percent LLO91–99-tetramer+ of CD8+ cells . | LLO91–99-tetramer+CD8+ cells/spleen . |
|---|---|---|
| Naive | <0.02 | <1,000 |
| + anti-CD4 mAb | <0.02 | <1,000 |
| L. monocytogenes | 0.06 ± 0.01 | 1,700 ± 100 |
| + anti-CD4 mAb | 0.07 ± 0.04 | 2,700 ± 1,600 |
| Gene gun | 0.09 ± 0.04 | 3,900 ± 2,800 |
| + anti-CD4 mAb | 0.10 ± 0.03 | 3,400 ± 900 |
Mice were infected with L. monocytogenes or immunized with pChly DNA using the gene gun. After 2 mo, mice were treated with anti-CD4 mAb or were left untreated. Injection of anti-CD4 mAb was repeated after 5 d, and 10 d after the first mAb injection mice were killed, spleen cells were counted, stained with anti-CD8 mAb, anti-CD62L mAb, and LLO91–99-tetramers, and analyzed by flow cytometry. Since in the spleen, a subpopulation of memory CD8+ T cells reacquires CD62L expression, results are given for total CD8+ T cells (CD62Llow and CD62Lhigh). Data represent mean ± SD of three mice per group. The experiment shown is representative for two individual experiments with similar results.