Table I.

Cells . | Reciprocal frequency ofgenome-positive cells (SD) ^{a}. | Percent of total spleen ^{b}. | Total number of cells ^{c}. | Latently infected cells ^{d}. |
---|---|---|---|---|

Resting B cells^{e} | 2,700 (1,600) | 45 | 9 × 10^{7} | 3.3 × 10^{4}^{f} |

Germinal center B cells^{g} | 8 (5) | 10.8 | 2.2 × 10^{7} | 2.7 × 10^{6} |

Cells . | Reciprocal frequency ofgenome-positive cells (SD) ^{a}. | Percent of total spleen ^{b}. | Total number of cells ^{c}. | Latently infected cells ^{d}. |
---|---|---|---|---|

Resting B cells^{e} | 2,700 (1,600) | 45 | 9 × 10^{7} | 3.3 × 10^{4}^{f} |

Germinal center B cells^{g} | 8 (5) | 10.8 | 2.2 × 10^{7} | 2.7 × 10^{6} |

Frequencies ± 95% confidence limits were determined by linear regression analysis of LDA-PCR data.

Percentage of each subset of total spleen cells was determined by FACS^{®} analysis.

Total number of cell subset per spleen based on an estimate of 2 × 10^{8} total cells/spleen 14 d after infection.

Number of latently infected cells based on the frequency of viral genome-positive cells within each cell type and its estimated total number per spleen.

Resting B cells were sorted as B220^{+} PNA^{low} with 98.15% purity. The contaminating fraction was 1.5% non-B cells and 0.03% germinal center B cells.

The 0.03% contamination with germinal center B cells, due to their high level of infection (one in eight), could account for one genome positive cell in every 3,413 purified resting B cells. Therefore, they could contribute as many as 3.2 × 10^{3} cells to the total number (3.3 × 10^{4}) of latently infected resting B cells.

Germinal center B cells were sorted as B220^{+} PNA^{high} with 98.64% purity. The contaminating fraction was 0.47% non-B cells and 0.83% resting B cells.

Data shown are the mean of three to four independent experiments, each analyzing pooled spleens from five to seven mice. Standard deviation values are shown between brackets.

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