Skip to Main Content
Table I.

MHV-68 Latency within Splenic B Cells During the Establishment of Latency (14 d after Infection) Is Mainly Established in Germinal Center B Cells


Cells

Reciprocal frequency ofgenome-positive cells (SD)a

Percent of total spleenb

Total number of cellsc

Latently infected cellsd
Resting B cellse 2,700 (1,600) 45 9 × 107 3.3 × 104f 
Germinal center B cellsg
 
8 (5)
 
10.8
 
2.2 × 107
 
2.7 × 106
 

Cells

Reciprocal frequency ofgenome-positive cells (SD)a

Percent of total spleenb

Total number of cellsc

Latently infected cellsd
Resting B cellse 2,700 (1,600) 45 9 × 107 3.3 × 104f 
Germinal center B cellsg
 
8 (5)
 
10.8
 
2.2 × 107
 
2.7 × 106
 
a

Frequencies ± 95% confidence limits were determined by linear regression analysis of LDA-PCR data.

b

Percentage of each subset of total spleen cells was determined by FACS® analysis.

c

Total number of cell subset per spleen based on an estimate of 2 × 108 total cells/spleen 14 d after infection.

d

Number of latently infected cells based on the frequency of viral genome-positive cells within each cell type and its estimated total number per spleen.

e

Resting B cells were sorted as B220+ PNAlow with 98.15% purity. The contaminating fraction was 1.5% non-B cells and 0.03% germinal center B cells.

f

The 0.03% contamination with germinal center B cells, due to their high level of infection (one in eight), could account for one genome positive cell in every 3,413 purified resting B cells. Therefore, they could contribute as many as 3.2 × 103 cells to the total number (3.3 × 104) of latently infected resting B cells.

g

Germinal center B cells were sorted as B220+ PNAhigh with 98.64% purity. The contaminating fraction was 0.47% non-B cells and 0.83% resting B cells.

Data shown are the mean of three to four independent experiments, each analyzing pooled spleens from five to seven mice. Standard deviation values are shown between brackets.

Close Modal

or Create an Account

Close Modal
Close Modal