Table II.

Cells . | Reciprocal frequency ofgenome-positive cells (SD) ^{a}. | Percent of total spleen ^{b}. | Total number of cells ^{c}. | Latently infected cells ^{d}. |
---|---|---|---|---|

Total B cells^{e} | 27,000 (4,600) | 42 | 2.5 × 10^{7} | 913 |

Naive B cells^{f} | 290,000 (150,000) | 36 | 2.2 × 10^{7} | 74^{g} |

Memory B cells^{h} | 3,100 (2,400) | 0.99 | 5.9 × 10^{5} | 189 |

Germinal center B cells^{i} | 1,100 (469) | 0.44 | 2.6 × 10^{5} | 233 |

Cells . | Reciprocal frequency ofgenome-positive cells (SD) ^{a}. | Percent of total spleen ^{b}. | Total number of cells ^{c}. | Latently infected cells ^{d}. |
---|---|---|---|---|

Total B cells^{e} | 27,000 (4,600) | 42 | 2.5 × 10^{7} | 913 |

Naive B cells^{f} | 290,000 (150,000) | 36 | 2.2 × 10^{7} | 74^{g} |

Memory B cells^{h} | 3,100 (2,400) | 0.99 | 5.9 × 10^{5} | 189 |

Germinal center B cells^{i} | 1,100 (469) | 0.44 | 2.6 × 10^{5} | 233 |

Frequencies ± 95% confidence limits were determined by linear regression analysis of LDA-PCR data.

Percentage of each subset of total spleen cells was determined by FACS^{®} analysis.

Total number of cell subset per spleen based on an estimate of 6 × 10^{7} cells/spleen.

Number of latently infected cells based on the frequency of viral genome-positive cells within each cell type and its estimated total number per spleen.

B cells were sorted as B220^{+} with 99.17% purity. The contaminating fraction was 0.75% non-B cells.

Naive B cells were sorted as IgD/M^{+} with 99% purity. The contaminating fraction was 0.8% non-B cells and 0.02% isotype-switched cells.

The 0.02% contamination with isotype-switched B cells, due to their high level of infection (1 in 2,100), could account for 1 genome-positive cell in every 5,004 purified resting B cells. Therefore, they would make a contribution of one to two cells to the total number (74) of latently infected naive B cells.

Memory B cells were sorted as isotype-switched (IgG_{1}^{+}, IgG_{2ab}^{+}, IgG_{3}^{+}_{,} IgA^{+}) CD38^{high} with 95% purity. The contaminating fraction was 2.23% nonisotype-switched B cells and 2.9% germinal center B cells.

Germinal center B cells were sorted as isotype-switched (IgG_{1}^{+}, IgG_{2ab}^{+}, IgG_{3}^{+}_{,} IgA^{+}) CD38^{low} with 96% purity. The contaminating fraction was 2.23% nonisotype-switched B cells and 1.9% memory B cells.

Data shown are the mean of three to four independent experiments, each analyzing pooled spleens from five to seven mice. Standard deviation values are shown between brackets.

This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.