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Table I.

FACS® Analysis of 3-mo-old (mean ± SEM)




B6

B6.Sle1

B6.lpr

B6.Sle1.lpr

P valuea


n = 8
n = 6
n = 6
n = 6
B6
B6.Sle1
B6.lpr
Spleen Weight (mg) 74.2 ± 10.6 89.6 ± 5.6 1 05.0 ± 9.3 413.2 ± 97.2 ** *** ** 
 Cell number (× 10−683.1 ± 13.7 107.2 ± 10.1 127.2 ± 29.1 224.6 ± 21.8 *** *** * 
 % CD4 T cell 15.4 ± 1.0 15.1 ± 0.8 16.7 ± 1.1 27.5 ± 0.6 *** *** *** 
 % CD8 T cell 10.2 ± 0.7 10.9 ± 0.5 9.6 ± 1.9 6.6 ± 0.6 * ** NS 
 % CD3+CD4CD8 (DN) 4.8 ± 1.1 8.2 ± 1.2 15.1 ± 2.2 15.9 ± 2.1 *** * NS 
 CD4 T cell: size (mean FSC) 46.1 ± 0.8 45.2 ± 0.9 50.2 ± 1.1 50.6 ± 1.0 ** ** NS 
 % CD69+ 27.1 ± 1.3 27.8 ± 0.6 35.1 ± 1.2 52.5 ± 4.6 *** ** * 
 % CD25+ 25.4 ± 2.8 28.2 ± 0.3 24.6 ± 2.0 42.4 ± 6.9 * NS NS 
 % CD62L−ve 51.9 ± 3.7 44.0 ± 3.8 72.2 ± 3.4 79.0 ± 0.7 ** ** NS 
 % CD45RB−ve 31.8 ± 4.3 38.2 ± 3.3 50.5 ± 3.6 81.4 ± 1.1 *** *** ** 
 CD8 T cell: % CD69+ 18.8 ± 2.6 18.6 ± 1.6 17.2 ± 0.5 47.5 ± 5.2 *** ** ** 
 % CD25+ 12.1 ± 2.0 14.8 ± 2.1 15.1 ± 3.9 37.0 ± 7.2 ** * NS 
 % CD62L−ve 52.4 ± 3.7 34.9 ± 4.5 65.6 ± 3.5 84.9 ± 2.7 *** *** * 
 % CD45RB−ve 10.6 ± 1.9 7.1 ± 0.3 20.7 ± 7.4 38.9 ± 6.4 ** *** NS 
 % B1a cells 4.9 ± 0.8 10.0 ± 4.7 19.9 ± 3.8 23.7 ± 4.0 *** *** * 
 % B2 cells 53.8 ± 3.3 52.1 ± 2.2 33.6 ± 6.8 16.8 ± 3.4 *** *** *** 
 B cell: size (mean FSC) 46.2 ± 0.8 48.2 ± 0.8 49.1 ± 0.7 51.5 ± 0.7 ** * NS 
 I-Ab (MFI)b 1,000 1,154 ± 55 1,163 ± 53 2,735 ± 125 *** *** *** 
 B7-1 (MFI)b 50 53.6 ± 9.4 57.7 ± 4.3 78.2 ± 3.9 * * * 
 B7-2 (MFI)b 50 59.8 ± 5.3 74.3 ± 14.6 93.0 ± 12.5 ** * NS 
 CD44 (MFI)b 300 303.3 ± 9.5 360.9 ± 32.6 570.9 ± 61.9 *** ** * 
LNc Weight (mg) 7.5 ± 1.1 13.6 ± 2.0 14.6 ± 2.8 111.0 ± 47.1 * ** ** 
 Cell number (× 1062.7 ± 0.4 4.3 ± 1.5 4.7 ± 1.2 17.3 ± 3.1 ** ** ** 
 % CD4 T cell 24.1 ± 2.2 22.5 ± 2.3 18.7 ± 2.2 17.7 ± 1.7 *** NS NS 
 % CD8 T cell 21.4 ± 2.0 23.4 ± 2.9 15.2 ± 2.2 8.7 ± 1.3 *** ** * 
 % CD3+CD4CD8 (DN) 10.9 ± 2.9 8.6 ± 1.4 13.4 ± 1.7 27.5 ± 3.2 ** *** * 
 CD4 T cell (mean FSC) 46.7 ± 1.7 45.4 ± 2.0 48.2 ± 1.4 52.4 ± 0.8 * * * 
 CD4 T cell: % CD69+ 23.8 ± 2.2 21.3 ± 1.2 35.0 ± 5.0 43.0 ± 2.9 *** *** NS 
 CD8 T cell: % CD69+ 15.2 ± 3.6 8.0 ± 1.1 23.3 ± 9.2 29.2 ± 2.9 * *** NS 
 % B1a cells 4.5 ± 0.4 4.7 ± 0.8 15.5 ± 5.1 35.1 ± 4.8 *** *** * 
 % B2 cells 34.4 ± 4.0 31.0 ± 2.2 37.0 ± 7.8 15.1 ± 3.2 ** ** NS 
 B cell size (mean FSC) 44.7 ± 1.7 45.5 ± 1.3 48.1 ± 1.3 53.5 ± 0.6 *** ** * 
PerC % B1a cells 37.6 ± 1.7 34.5 ± 3.4 38.1 ± 6.1 61.1 ± 4.8 *** *** * 

 
% B2 cells
 
61.7 ± 0.8
 
56.4 ± 1.3
 
55.6 ± 5.3
 
22.3 ± 0.9
 
***
 
***
 
**
 



B6

B6.Sle1

B6.lpr

B6.Sle1.lpr

P valuea


n = 8
n = 6
n = 6
n = 6
B6
B6.Sle1
B6.lpr
Spleen Weight (mg) 74.2 ± 10.6 89.6 ± 5.6 1 05.0 ± 9.3 413.2 ± 97.2 ** *** ** 
 Cell number (× 10−683.1 ± 13.7 107.2 ± 10.1 127.2 ± 29.1 224.6 ± 21.8 *** *** * 
 % CD4 T cell 15.4 ± 1.0 15.1 ± 0.8 16.7 ± 1.1 27.5 ± 0.6 *** *** *** 
 % CD8 T cell 10.2 ± 0.7 10.9 ± 0.5 9.6 ± 1.9 6.6 ± 0.6 * ** NS 
 % CD3+CD4CD8 (DN) 4.8 ± 1.1 8.2 ± 1.2 15.1 ± 2.2 15.9 ± 2.1 *** * NS 
 CD4 T cell: size (mean FSC) 46.1 ± 0.8 45.2 ± 0.9 50.2 ± 1.1 50.6 ± 1.0 ** ** NS 
 % CD69+ 27.1 ± 1.3 27.8 ± 0.6 35.1 ± 1.2 52.5 ± 4.6 *** ** * 
 % CD25+ 25.4 ± 2.8 28.2 ± 0.3 24.6 ± 2.0 42.4 ± 6.9 * NS NS 
 % CD62L−ve 51.9 ± 3.7 44.0 ± 3.8 72.2 ± 3.4 79.0 ± 0.7 ** ** NS 
 % CD45RB−ve 31.8 ± 4.3 38.2 ± 3.3 50.5 ± 3.6 81.4 ± 1.1 *** *** ** 
 CD8 T cell: % CD69+ 18.8 ± 2.6 18.6 ± 1.6 17.2 ± 0.5 47.5 ± 5.2 *** ** ** 
 % CD25+ 12.1 ± 2.0 14.8 ± 2.1 15.1 ± 3.9 37.0 ± 7.2 ** * NS 
 % CD62L−ve 52.4 ± 3.7 34.9 ± 4.5 65.6 ± 3.5 84.9 ± 2.7 *** *** * 
 % CD45RB−ve 10.6 ± 1.9 7.1 ± 0.3 20.7 ± 7.4 38.9 ± 6.4 ** *** NS 
 % B1a cells 4.9 ± 0.8 10.0 ± 4.7 19.9 ± 3.8 23.7 ± 4.0 *** *** * 
 % B2 cells 53.8 ± 3.3 52.1 ± 2.2 33.6 ± 6.8 16.8 ± 3.4 *** *** *** 
 B cell: size (mean FSC) 46.2 ± 0.8 48.2 ± 0.8 49.1 ± 0.7 51.5 ± 0.7 ** * NS 
 I-Ab (MFI)b 1,000 1,154 ± 55 1,163 ± 53 2,735 ± 125 *** *** *** 
 B7-1 (MFI)b 50 53.6 ± 9.4 57.7 ± 4.3 78.2 ± 3.9 * * * 
 B7-2 (MFI)b 50 59.8 ± 5.3 74.3 ± 14.6 93.0 ± 12.5 ** * NS 
 CD44 (MFI)b 300 303.3 ± 9.5 360.9 ± 32.6 570.9 ± 61.9 *** ** * 
LNc Weight (mg) 7.5 ± 1.1 13.6 ± 2.0 14.6 ± 2.8 111.0 ± 47.1 * ** ** 
 Cell number (× 1062.7 ± 0.4 4.3 ± 1.5 4.7 ± 1.2 17.3 ± 3.1 ** ** ** 
 % CD4 T cell 24.1 ± 2.2 22.5 ± 2.3 18.7 ± 2.2 17.7 ± 1.7 *** NS NS 
 % CD8 T cell 21.4 ± 2.0 23.4 ± 2.9 15.2 ± 2.2 8.7 ± 1.3 *** ** * 
 % CD3+CD4CD8 (DN) 10.9 ± 2.9 8.6 ± 1.4 13.4 ± 1.7 27.5 ± 3.2 ** *** * 
 CD4 T cell (mean FSC) 46.7 ± 1.7 45.4 ± 2.0 48.2 ± 1.4 52.4 ± 0.8 * * * 
 CD4 T cell: % CD69+ 23.8 ± 2.2 21.3 ± 1.2 35.0 ± 5.0 43.0 ± 2.9 *** *** NS 
 CD8 T cell: % CD69+ 15.2 ± 3.6 8.0 ± 1.1 23.3 ± 9.2 29.2 ± 2.9 * *** NS 
 % B1a cells 4.5 ± 0.4 4.7 ± 0.8 15.5 ± 5.1 35.1 ± 4.8 *** *** * 
 % B2 cells 34.4 ± 4.0 31.0 ± 2.2 37.0 ± 7.8 15.1 ± 3.2 ** ** NS 
 B cell size (mean FSC) 44.7 ± 1.7 45.5 ± 1.3 48.1 ± 1.3 53.5 ± 0.6 *** ** * 
PerC % B1a cells 37.6 ± 1.7 34.5 ± 3.4 38.1 ± 6.1 61.1 ± 4.8 *** *** * 

 
% B2 cells
 
61.7 ± 0.8
 
56.4 ± 1.3
 
55.6 ± 5.3
 
22.3 ± 0.9
 
***
 
***
 
**
 
a

Student's t test was performed to compare all three control strains with B6.Sle1|lpr mice. For the comparison of LN weights, Mann-Whitney Rank Sum test was employed, as these data sets failed normality tests.

*

P < 0.05;

**

P < 0.01; and

***

P < 0.001. NS, not significant (P > 0.05).

b

Each FACS® staining experiment was conducted with B6 controls and the experimental strains in parallel. Within each experiment, the level of expression of I-Ab, B7-1, B7-2, and CD44 on B6 B cells was arbitrarily set at 1,000, 50, 50, and 300 MFI units, respectively, by adjusting the fluorescence channel voltage. This facilitated the comparison of staining experiments done on different days. MFI, mean fluorescence intensity.

c

Shown data was obtained from individual inguinal LNs isolated from the different strains.

Cellular composition of B6.Sle1|lpr and control spleens, LNs, and PerC. Six to eight mice of each strain were examined at 3 mo of age. Shown values represent mean ± SEM. For the spleen and LNs, the shown percentages of CD4, CD8, DN, B1a, and B2 cells represent percentages of all lymphocytes, whereas the percentages of B1a and B2 cells in the PerC represent percentages of all PerC B cells.

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