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Table II.

Clonogenic Progenitor Assay Reveals a Differentiation Block in Myeloid Progenitors


BMa

No. of colonies per 105 cellsb

Mean distribution of mature colonies (%)

Immature
Mature
CFU-Mix
CFU-GM
CFU-G
CFU-M
BFU-E
AEpos 196 ± 60 17 ± 7 6.1 19.3 7.2 42.0 24.2 
GFPpos 49 ± 22 2.3 14.9 20.15 29.4 33.8 
Unsortedc
 
0
 
50 ± 28
 
8.2
 
15.2
 
17.7
 
29.4
 
31.2
 

BMa

No. of colonies per 105 cellsb

Mean distribution of mature colonies (%)

Immature
Mature
CFU-Mix
CFU-GM
CFU-G
CFU-M
BFU-E
AEpos 196 ± 60 17 ± 7 6.1 19.3 7.2 42.0 24.2 
GFPpos 49 ± 22 2.3 14.9 20.15 29.4 33.8 
Unsortedc
 
0
 
50 ± 28
 
8.2
 
15.2
 
17.7
 
29.4
 
31.2
 
a

BM was isolated from femurs and tibiae and then either sorted for eGFP expression or assayed directly.

b

The mean value and SD from three independent mice for each group performed in triplicate at two different cell concentrations are presented.

c

Unsorted cells from FMEV-GFP–infected transplants.

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