Substrate Specificity of the Recombinant TcOYE
Substrate . | Cofactor . | Km . | Vmax/specific activity . |
|---|---|---|---|
| . | (100 μM) . | (μM) . | (nmol/min/mg) . |
| 9,11-endoperoxide PGH2 | NADH | — | 554 |
| NADPH | 5.0 | 766 | |
| Hydrogen peroxide | NADPH | 2.3 | 99 |
| BHPa | NADPH | ND | 282 |
| Menadione | NADH | — | 499 |
| NADPH | 0.82 | 700 | |
| β-lapachone | NADH | 0.17 | 650 |
| NADPH | — | 433 | |
| 4-nitroquinoline-N-oxide | NADH | — | 759 |
| NADPH | 9.5 | 1,110 | |
| Nifurtimox | NADH | — | 290 |
| NADPH | 19.0 | 353 | |
| Phenazine methosulfateb | NADPH | 10.4 | 235 |
| Mevinolinc | NADH | ND | 555 |
| 12-oxo phytodienoic acidd | NADPH | ND | 152 |
| 9-oxo ODEe | NADPH | ND | 54 |
| Econazolef | NADH | ND | 43 |
| Benznidazole | — | ND | n.d. |
| Miconazoleg | — | ND | n.d. |
| Ketoconazoleh | — | ND | n.d. |
| Crystal violeti | — | ND | n.d. |
| BHTj | — | ND | n.d. |
| BHAk | — | ND | n.d. |
Substrate . | Cofactor . | Km . | Vmax/specific activity . |
|---|---|---|---|
| . | (100 μM) . | (μM) . | (nmol/min/mg) . |
| 9,11-endoperoxide PGH2 | NADH | — | 554 |
| NADPH | 5.0 | 766 | |
| Hydrogen peroxide | NADPH | 2.3 | 99 |
| BHPa | NADPH | ND | 282 |
| Menadione | NADH | — | 499 |
| NADPH | 0.82 | 700 | |
| β-lapachone | NADH | 0.17 | 650 |
| NADPH | — | 433 | |
| 4-nitroquinoline-N-oxide | NADH | — | 759 |
| NADPH | 9.5 | 1,110 | |
| Nifurtimox | NADH | — | 290 |
| NADPH | 19.0 | 353 | |
| Phenazine methosulfateb | NADPH | 10.4 | 235 |
| Mevinolinc | NADH | ND | 555 |
| 12-oxo phytodienoic acidd | NADPH | ND | 152 |
| 9-oxo ODEe | NADPH | ND | 54 |
| Econazolef | NADH | ND | 43 |
| Benznidazole | — | ND | n.d. |
| Miconazoleg | — | ND | n.d. |
| Ketoconazoleh | — | ND | n.d. |
| Crystal violeti | — | ND | n.d. |
| BHTj | — | ND | n.d. |
| BHAk | — | ND | n.d. |
t-Butyl hydroperoxide
5-Methyl-phenazinium methyl sulfate
2β,6α-dimethyl-8α-(2-methyl-1-oxobutoxy)-mevinic acid lactone
4-oxo-5β-(2Z-pentenyl)-2-cyclopentene-1β-octanoic acid
9-oxo-10E, 12Z-octadecadienoic acid
1-[2-([4-Chlorophenyl]methoxy)-2-(2,4-dichlorophenyl)ethyl-1H-imidazole
1-[2,4-Dichloro-β-([2,4-dichlorobenzyl]-oxy)phenethyl]imidazole
cis-1-Acetyl-4-[4-[[2-(2,4-dichlorophenyl)-2-(1H-imidazol-1- ylmethyl)-1,3-dioxolan-4-yl-methoxy]phenyl]piperazine
N-[4-[Bis[4-(dimethylamino)-phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-methanaminium chloride
(2,6-Di-tert-butyl-p-cresol)
[2(3)-tert-butyl-4-hydroxianisole]
n.d., not detected.