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Table I.

Coexpression Analysis of Cytokines and ICOS in CD4+ T Cells


pLN CD4+ T cells ex vivo

TNF-α

IL-3

IL-6

IFN-γ

IL-2

GM-CSF

IL-13

IL-4

IL-5

IL-10
Total frequency of cells secreting a
   given cytokinea 58.7% 1.0% 0.13% 4.8% 3.8% 0.9% 1.5% 1.2% 0.35% 0.7% 
Proportion of ICOS+ cells in the population
   of CD4+ T cells secreting a given cytokinea
 
9%
 
22%
 
21%
 
29%
 
23%
 
42%
 
56%
 
57%
 
58%
 
46%
 

pLN CD4+ T cells ex vivo

TNF-α

IL-3

IL-6

IFN-γ

IL-2

GM-CSF

IL-13

IL-4

IL-5

IL-10
Total frequency of cells secreting a
   given cytokinea 58.7% 1.0% 0.13% 4.8% 3.8% 0.9% 1.5% 1.2% 0.35% 0.7% 
Proportion of ICOS+ cells in the population
   of CD4+ T cells secreting a given cytokinea
 
9%
 
22%
 
21%
 
29%
 
23%
 
42%
 
56%
 
57%
 
58%
 
46%
 
a

pLN cells were activated with PMA and ionomycin for 5 h in the presence of Brefeldin A for the last 3 h and analyzed for intracellular cytokines by flow cytometry.

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