Substrate Voltage Dependence Normalized to Km at −100 mV
V (mV) . | . | −80 . | . | −60 . | . | −40 . | . | −20 . | . | 0 . |
---|---|---|---|---|---|---|---|---|---|---|
KmNa (0.1 mM Pi) | 1.01 | 1.08 | 1.17 | 1.41 | 1.48 | |||||
KmNa (1.0 mM Pi) | 1.00 | 1.04 | 1.08 | 1.21 | 1.24 | |||||
KmPi (50 mM Na+) | 1.14 | 1.57 | 2.08 | 2.41 | 3.68 | |||||
KmPi (96 mM Na+) | 1.03 | 1.14 | 1.28 | 1.43 | 1.59 |
V (mV) . | . | −80 . | . | −60 . | . | −40 . | . | −20 . | . | 0 . |
---|---|---|---|---|---|---|---|---|---|---|
KmNa (0.1 mM Pi) | 1.01 | 1.08 | 1.17 | 1.41 | 1.48 | |||||
KmNa (1.0 mM Pi) | 1.00 | 1.04 | 1.08 | 1.21 | 1.24 | |||||
KmPi (50 mM Na+) | 1.14 | 1.57 | 2.08 | 2.41 | 3.68 | |||||
KmPi (96 mM Na+) | 1.03 | 1.14 | 1.28 | 1.43 | 1.59 |
Data are taken from the absolute apparent affinity constant data for Pi binding and Na+ binding in Figs. 2,C and 3 C, respectively.