Skip to Main Content
Table I.

Detection of CD80 and CD86 on Freshly Obtained Ocular Tissues


Tissues

CD80 (B7-1)

CD86 (B7-2)
Corneal epithelium 
Corneal stroma (keratocytes) − − 
Corneal endothelium − − 
Iris 
Ciliary body 
Lens epithelium 
Lens fiber cells − − 
Neural retina − − 
RPE
 
+
 
+
 

Tissues

CD80 (B7-1)

CD86 (B7-2)
Corneal epithelium 
Corneal stroma (keratocytes) − − 
Corneal endothelium − − 
Iris 
Ciliary body 
Lens epithelium 
Lens fiber cells − − 
Neural retina − − 
RPE
 
+
 
+
 

Eyes of BALB/c mice were enucleated, snap frozen, sectioned, placed on glass slides, fixed with acetone, and stained with antibodies to CD80, CD86, and isotype IgG controls, followed by biotin-conjugated sec-ondary anti-antibodies, and then developed with streptavidin/horse-radish peroxidase and the DAB substrate. Specimens were examined by light microscopy. Staining of tissues and cells with DAB is indicated with +.

Close Modal

or Create an Account

Close Modal
Close Modal