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Table II.

Detection of CD80 and CD86 on Cultured Ocular PE Cells


PE cell types

CD80 (B7-1)

CD86 (B7-2)
Iris 
Iris, treated with IFNγ ++ ++ 
Ciliary body  − 
Ciliary body, treated with IFNγ ++ 
Retina (+)a 
Retina, treated with IFNγ
 
++
 
(+)a
 

PE cell types

CD80 (B7-1)

CD86 (B7-2)
Iris 
Iris, treated with IFNγ ++ ++ 
Ciliary body  − 
Ciliary body, treated with IFNγ ++ 
Retina (+)a 
Retina, treated with IFNγ
 
++
 
(+)a
 
a

Staining within cytoplasmic granules without evidence of cell surface staining.

Extent of staining is presented semiquantitatively as −, +, or ++.

PE cells from iris, ciliary body, and retina were cultured for 14 d, washed, and then incubated overnight in serum-free medium, with or without 100 U/ml IFNγ. The cells were then removed, fixed with acetone, and stained with FITC-conjugated anti-CD80 or anti-CD86 antibodies, and examined by fluorescent microscopy.

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