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Table I.

Frequency of DC Subsets After Immunization


DC
 subset

No
 injection

PBS

CpG
 ODN

LACK

LACK +
 CpG ODN
B220+ 56.6 ± 4.5 36.6 ± 5.6 16.4 ± 6.6 26.5 ± 7.1 17.7 ± 9.0 
CD8+ 3.7 ± 0.4 4.0 ± 1.8 4.8 ± 2.9 4.7 ± 2.1 6.7 ± 5.6 
CD8
 
39.8 ± 4.0
 
58.8 ± 5.0
 
78.8 ± 5.4
 
68.9 ± 8.7
 
75.7 ± 9.3
 

DC
 subset

No
 injection

PBS

CpG
 ODN

LACK

LACK +
 CpG ODN
B220+ 56.6 ± 4.5 36.6 ± 5.6 16.4 ± 6.6 26.5 ± 7.1 17.7 ± 9.0 
CD8+ 3.7 ± 0.4 4.0 ± 1.8 4.8 ± 2.9 4.7 ± 2.1 6.7 ± 5.6 
CD8
 
39.8 ± 4.0
 
58.8 ± 5.0
 
78.8 ± 5.4
 
68.9 ± 8.7
 
75.7 ± 9.3
 

The frequency of CD11c+CD8+B220, CD11c+CD8B220, and CD11c+B220+ cells from draining LN of naive and immunized mice was determined from four independent experiments. Results are expressed as geometric mean ± SEM: P < 0.05 when comparing any of the immunization groups to naive mice; P < 0.05 when comparing mice immunized with LACK protein, CpG ODN, or both with PBS-injected mice.

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