Protease Activities in Ear Tissue Extracts
Source of tissue . | Chymotrypsin-like activity mOD/(min × mg tissue) . | Trypsin-like activity mOD/(min × mg tissue) . | CPA activity mOD/(min × mg tissue) . |
|---|---|---|---|
| mMCP-4+/+ mice | 0.52 ± 0.044 | 2.3 ± 0.31 | −0.47 ± 0.11 |
| (n = 2) | (n = 2) | (n = 2) | |
| mMCP-4−/− mice | n.d. | 2.8 ± 0.87 | −0.44 ± 0.073 |
| (n = 2) | (n = 2) | (n = 2) | |
| mMCP-4+/− mice | 0.21 ± 0.015 | 3.7 ± 0.57 | −0.45 ± 0.064 |
| (n = 2) | (n = 2) | (n = 2) |
Source of tissue . | Chymotrypsin-like activity mOD/(min × mg tissue) . | Trypsin-like activity mOD/(min × mg tissue) . | CPA activity mOD/(min × mg tissue) . |
|---|---|---|---|
| mMCP-4+/+ mice | 0.52 ± 0.044 | 2.3 ± 0.31 | −0.47 ± 0.11 |
| (n = 2) | (n = 2) | (n = 2) | |
| mMCP-4−/− mice | n.d. | 2.8 ± 0.87 | −0.44 ± 0.073 |
| (n = 2) | (n = 2) | (n = 2) | |
| mMCP-4+/− mice | 0.21 ± 0.015 | 3.7 ± 0.57 | −0.45 ± 0.064 |
| (n = 2) | (n = 2) | (n = 2) |
Extracts were prepared from ear tissue and were assayed for trypsin-like, chymotrypsin-like, and CPA activities as described in Materials and Methods. Note that hydrolysis of the CPA substrate results in decreased absorbance. n.d., none detected.