Rockefeller University Press

TABLE III

Kinetics of Binding and Gating in AChRs Activated by Choline

Construct	k₊	k₋	K_d	β₂	α₂	L₂
	mM⁻¹ s⁻¹	s⁻¹	mM	s⁻¹	s⁻¹
wt	—	—	2	100	2,100	0.047
αD97H	1,055 ± 40	2,993 ± 141	2.84 ± 0.24	3,189 ± 168	2,759 ± 149	1.15 ± 0.14
αD97E	2,839 ± 187	6,070 ± 467	2.14 ± 0.32	3,024 ± 131	2,293 ± 214	1.31 ± 0.21

Construct	k₊	k₋	K_d	β₂	α₂	L₂
	mM⁻¹ s⁻¹	s⁻¹	mM	s⁻¹	s⁻¹
wt	—	—	2	100	2,100	0.047
αD97H	1,055 ± 40	2,993 ± 141	2.84 ± 0.24	3,189 ± 168	2,759 ± 149	1.15 ± 0.14
αD97E	2,839 ± 187	6,070 ± 467	2.14 ± 0.32	3,024 ± 131	2,293 ± 214	1.31 ± 0.21

The binding and gating rate constants (mean ± SD) were estimated by jointly fitting interval durations from different concentrations of choline (one patch each at 1, 2 and 5 mM) using Scheme I (see Fig. 4). K_d was calculated as the ratio k₋/k₊. The K_d value for choline in wt receptors is from (Zhou et al., 1999).

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