Unliganded Gating Parameters of Wild-type and αD97 Mutants
α97 construct . | Opening frequency . | Open probability (×104) . | τo . | n . |
|---|---|---|---|---|
| s−1 | ms | |||
| wt | 0.10 ± 0.01 | 0.07 ± 0.01 | 0.07 ± 0.02 | 3 |
| H | 1.5 ± 0.54 | 1.02 ± 0.23 | 0.08 ± 0.01 | 3 |
| E | 8.81 ± 2.66 | 12.5 ± 4.87 | 0.14 ± 0.03 | 4 |
| F | 3.29 ± 0.82 | 1.97 ± 0.30 | 0.06 ± 0.01 | 4 |
| Q | 6.90 ± 1.61 | 5.61 ± 2.92 | 0.08 ± 0.01 | 4 |
| R | 9.55 ± 1.35 | 10.3 ± 1.82 | 0.10 ± 0.01 | 3 |
| A | 33.2 ± 11.4 | 34.6 ± 12.9 | 0.10 ± 0.01 | 6 |
α97 construct . | Opening frequency . | Open probability (×104) . | τo . | n . |
|---|---|---|---|---|
| s−1 | ms | |||
| wt | 0.10 ± 0.01 | 0.07 ± 0.01 | 0.07 ± 0.02 | 3 |
| H | 1.5 ± 0.54 | 1.02 ± 0.23 | 0.08 ± 0.01 | 3 |
| E | 8.81 ± 2.66 | 12.5 ± 4.87 | 0.14 ± 0.03 | 4 |
| F | 3.29 ± 0.82 | 1.97 ± 0.30 | 0.06 ± 0.01 | 4 |
| Q | 6.90 ± 1.61 | 5.61 ± 2.92 | 0.08 ± 0.01 | 4 |
| R | 9.55 ± 1.35 | 10.3 ± 1.82 | 0.10 ± 0.01 | 3 |
| A | 33.2 ± 11.4 | 34.6 ± 12.9 | 0.10 ± 0.01 | 6 |
The opening frequency (per patch; mean ± SEM) was estimated as the inverse of mean duration of closed intervals (τc). The open probability (per patch) was calculated as the τo/(τo + τc), where τo is the mean duration of open intervals; n is the number of patches. The effect of the mutation on the opening frequency correlates with its effect on L2 (Fig. 7).