Rockefeller University Press

TABLE VII

Rate and Equilibrium Constants of Loop 5 Deletion Mutants Activated by ACh

Construct	k₊	k₋₁	K_d	β₂	α₂	L₂
	μM⁻¹ s⁻¹	s⁻¹		s⁻¹	s⁻¹
wt	167 ± 2	24,745 ± 257	148	50,000	2,100	23.8
Del 97	42 ± 4	15,099 ± 1,185	359	3,928 ± 132	6,704 ± 744	0.59
Del 97-98	63 ± 2	5,008 ± 135	79	7,715 ± 174	4,045 ± 131	1.91

Construct	k₊	k₋₁	K_d	β₂	α₂	L₂
	μM⁻¹ s⁻¹	s⁻¹		s⁻¹	s⁻¹
wt	167 ± 2	24,745 ± 257	148	50,000	2,100	23.8
Del 97	42 ± 4	15,099 ± 1,185	359	3,928 ± 132	6,704 ± 744	0.59
Del 97-98	63 ± 2	5,008 ± 135	79	7,715 ± 174	4,045 ± 131	1.91

The binding and gating rate constants were estimated by jointly fitting interval durations elicited by different concentrations of ACh, using Scheme I (see Fig. 11). K_d was calculated as the ratio k₋/k₊. Wild type values are from (Salamone et al., 1999).

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