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Table I.

Cytokine Production of Tg T Cell Lines



IFN-γ

TNF-α

IL-10

IL-4
Tg T cell line
Mu
Hu
Mu
Hu
Mu
Hu
Mu
Hu
MS2-3C8(B6) 26.6 52.6 ± 1.1 4.2 ± 0.1 7.0 ± 0.2 0.1 0.1 0.6 0.8 
MS2-3C8(G2) 22.2 ± 1.8 50.8 ± 7.1 2.8 ± 0.1 4.8 ± 0.3 1.0 1.3 1.3 1.8 
HD4-1C2
 
0
 
156.4 ± 7.2
 
0
 
4.8 ± 0.7
 
0
 
0.3
 
0
 
0.8
 


IFN-γ

TNF-α

IL-10

IL-4
Tg T cell line
Mu
Hu
Mu
Hu
Mu
Hu
Mu
Hu
MS2-3C8(B6) 26.6 52.6 ± 1.1 4.2 ± 0.1 7.0 ± 0.2 0.1 0.1 0.6 0.8 
MS2-3C8(G2) 22.2 ± 1.8 50.8 ± 7.1 2.8 ± 0.1 4.8 ± 0.3 1.0 1.3 1.3 1.8 
HD4-1C2
 
0
 
156.4 ± 7.2
 
0
 
4.8 ± 0.7
 
0
 
0.3
 
0
 
0.8
 

Splenic MS2-3C8 and HD4-1C2 Th1 cell lines were confirmed by high IFN-γ and TNF-α production after culture with 1 μg/ml of human MBP 111–129 (Hu) or murine MBP 111–129 (Mu) for 72 h. The unit of cytokine production is indicated as ng/ml ± SD.

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