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Table I.

Hematopoietic Cell Chimerism After Human USSC in Utero Transplantation into Fetal Sheep


Antibody

Blood
 4 moapost-TPX

Bone marrow
 4 moapost-TPX

Bone marrow
 5 mob post-TPX

Bone marrow
 12 mob post-TPX
CD45 1.66 (0.06–4.68) 0.09 (0.06–0.12) 1.42 (1.10–1.90) 2.75 (1.80–4.20) 
CD34 0.00 (0.00–0.00) 0.14 (0.00–0.40) 0.09 (0.07–0.14) 0.18 (0.13–0.20) 
Glycophorin A 4.45 (2.63–6.50) 2.26 (1.72–2.67) 3.02 (1.20–5.20) 2.30 (0.00–4.20) 
CD7 0.59 (0.41–0.78) 2.23 (0.95–4.04) 1.85 (0.90–3.00) 2.70 (1.10–4.90) 
CD3 0.44 (0.00–0.89) 0.29 (0.00–0.89) 0.37 (0.00–0.90) 1.08 (0.90–1.30) 
HLA-DR 3.07 (0.67–6.68) 0.35 (0.00–1.05) ND ND 
CD10 0.15 (0.08–0.29) 0.06 (0.00–0.14) ND ND 
CD33 0.00 (0.00–0.00) 0.01 (0.00–0.04) ND 0.70 (0.00–1.40) 
CD13 0.06 (0.00–0.15) 0.01 (0.00–0.02) ND ND 
CD20
 
0.00 (0.00–0.00)
 
0.00 (0.00–0.00)
 
0.15 (0.00–0.30)
 
0.52 (0.00–0.60)
 

Antibody

Blood
 4 moapost-TPX

Bone marrow
 4 moapost-TPX

Bone marrow
 5 mob post-TPX

Bone marrow
 12 mob post-TPX
CD45 1.66 (0.06–4.68) 0.09 (0.06–0.12) 1.42 (1.10–1.90) 2.75 (1.80–4.20) 
CD34 0.00 (0.00–0.00) 0.14 (0.00–0.40) 0.09 (0.07–0.14) 0.18 (0.13–0.20) 
Glycophorin A 4.45 (2.63–6.50) 2.26 (1.72–2.67) 3.02 (1.20–5.20) 2.30 (0.00–4.20) 
CD7 0.59 (0.41–0.78) 2.23 (0.95–4.04) 1.85 (0.90–3.00) 2.70 (1.10–4.90) 
CD3 0.44 (0.00–0.89) 0.29 (0.00–0.89) 0.37 (0.00–0.90) 1.08 (0.90–1.30) 
HLA-DR 3.07 (0.67–6.68) 0.35 (0.00–1.05) ND ND 
CD10 0.15 (0.08–0.29) 0.06 (0.00–0.14) ND ND 
CD33 0.00 (0.00–0.00) 0.01 (0.00–0.04) ND 0.70 (0.00–1.40) 
CD13 0.06 (0.00–0.15) 0.01 (0.00–0.02) ND ND 
CD20
 
0.00 (0.00–0.00)
 
0.00 (0.00–0.00)
 
0.15 (0.00–0.30)
 
0.52 (0.00–0.60)
 
a

n = 3 animals were analyzed; mean (range) values in percentages are presented.

b

n = 4 animals were analyzed; mean values in percentages are presented.

Each fetus was transplanted with 1,500 cells. Please note that the values for some markers together (HLA-DR; CD7, CD3) exceed those of CD45. The up and down regulation of CD45 on different cell populations in this xenogeneic model was described previously (49). ND, not done.

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