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Table II.

Summary of Single Cell PCR Analysis of Micromanipulated Cells Revealing Complete Absence of Cell Fusion Events in This Noninjury Model


Gene locus

Tissue

Staining

Positive cells

Buffer controls
Human VHHuman Positive 8/24  
Human VHChimera Negative 0/24  
Human VHChimera Positive 22/80 0/16 
Human TCRVβ7.2 Human Positive 8/24  
Human TCRVβ7.2 Chimera Negative 0/24  
Human TCRVβ7.2 Chimera Positive 21/80 0/16 
Σ Human Positive 8/24  
Human VH1 or TCRVβ7.2 Chimera Negative 0/24  
 Chimera Positive 24/80 0/16 
Ovine VHHuman Positive 0/24  
Ovine VHChimera Negative 9/24  
Ovine VHChimera Positive 0/80 0/16 
Ovine TCRCδ Human Positive 0/24  
Ovine TCRCδ Chimera Negative 9/24  
Ovine TCRCδ Chimera Positive 0/80 0/16 
Σ Human Positive 0/24  
Ovine VH7 or TCRCδ Chimera Negative 9/24  

 
Chimera
 
Positive
 
0/80
 
0/16
 

Gene locus

Tissue

Staining

Positive cells

Buffer controls
Human VHHuman Positive 8/24  
Human VHChimera Negative 0/24  
Human VHChimera Positive 22/80 0/16 
Human TCRVβ7.2 Human Positive 8/24  
Human TCRVβ7.2 Chimera Negative 0/24  
Human TCRVβ7.2 Chimera Positive 21/80 0/16 
Σ Human Positive 8/24  
Human VH1 or TCRVβ7.2 Chimera Negative 0/24  
 Chimera Positive 24/80 0/16 
Ovine VHHuman Positive 0/24  
Ovine VHChimera Negative 9/24  
Ovine VHChimera Positive 0/80 0/16 
Ovine TCRCδ Human Positive 0/24  
Ovine TCRCδ Chimera Negative 9/24  
Ovine TCRCδ Chimera Positive 0/80 0/16 
Σ Human Positive 0/24  
Ovine VH7 or TCRCδ Chimera Negative 9/24  

 
Chimera
 
Positive
 
0/80
 
0/16
 
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