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Table I.

Results of single GMP cultures




Pure eosinophils

Neutrophils and macrophages

Neutrophils

Macrophages

Total
No eosinophils NA 240 682 162 1,084 
Eosinophil-containing wells 14 
Total
 
6
 
244
 
686
 
162
 
1,098
 



Pure eosinophils

Neutrophils and macrophages

Neutrophils

Macrophages

Total
No eosinophils NA 240 682 162 1,084 
Eosinophil-containing wells 14 
Total
 
6
 
244
 
686
 
162
 
1,098
 

Total 1,200 single GMPs were cultured in the presence of Slf, IL-3, GM-CSF, IL-5, and IL-9. Plating efficiency was 91% (1,098/1,200). Each colony type was determined by May-Grünwald Giemsa (MGG) staining. In case eosinophils were detected in a colony, EoPO transcripts were additionally analyzed by RT-PCR.

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