Vβ-specific expansion of Vα14tg.CD1d−/− DN T cells stimulated by lipid antigens
| . | α-GalCer (ng/ml) . | . | . | . | iGb3 (ng/ml) . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.01 | 0.1 | 1 | 10 | 0.5 | 5 | 50 | 500 | |||||||
| Vβ2 | 0.6 | 0.9 | 4.2 | 9.2 | 0.5 | 0.7 | 3.7 | 4.3 | ||||||
| Vβ6 | ND | ND | 1.7 | 2.7 | ND | ND | <0.5 | <0.5 | ||||||
| Vβ7 | 2.1 | 4.0 | 15.6 | 27.3 | 1.4 | 3.0 | 10.2 | 14.6 | ||||||
| Vβ8.1 + 8.2 | 1.2 | 1.9 | 8.7 | 17.3 | 0.6 | 1.2 | 5.1 | 6.8 | ||||||
| Vβ8.2 | 1.5 | 2.6 | 12.0 | 23.1 | 0.7 | 1.3 | 6.0 | 7.2 | ||||||
| Vβ9 | ND | ND | <0.5 | 4.7 | ND | ND | <0.5 | <0.5 | ||||||
| Vβ10 | ND | ND | <0.5 | 4.1 | ND | ND | <0.5 | <0.5 | ||||||
| Vβ14 | ND | ND | 3.2 | 9.4 | ND | ND | <0.5 | <0.5 | ||||||
| non-Vβ2, 7, & 8 | ND | ND | 0.6 | 0.7 | ||||||||||
| . | α-GalCer (ng/ml) . | . | . | . | iGb3 (ng/ml) . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.01 | 0.1 | 1 | 10 | 0.5 | 5 | 50 | 500 | |||||||
| Vβ2 | 0.6 | 0.9 | 4.2 | 9.2 | 0.5 | 0.7 | 3.7 | 4.3 | ||||||
| Vβ6 | ND | ND | 1.7 | 2.7 | ND | ND | <0.5 | <0.5 | ||||||
| Vβ7 | 2.1 | 4.0 | 15.6 | 27.3 | 1.4 | 3.0 | 10.2 | 14.6 | ||||||
| Vβ8.1 + 8.2 | 1.2 | 1.9 | 8.7 | 17.3 | 0.6 | 1.2 | 5.1 | 6.8 | ||||||
| Vβ8.2 | 1.5 | 2.6 | 12.0 | 23.1 | 0.7 | 1.3 | 6.0 | 7.2 | ||||||
| Vβ9 | ND | ND | <0.5 | 4.7 | ND | ND | <0.5 | <0.5 | ||||||
| Vβ10 | ND | ND | <0.5 | 4.1 | ND | ND | <0.5 | <0.5 | ||||||
| Vβ14 | ND | ND | 3.2 | 9.4 | ND | ND | <0.5 | <0.5 | ||||||
| non-Vβ2, 7, & 8 | ND | ND | 0.6 | 0.7 | ||||||||||
The fold increase in the absolute number of cells expressing indicated Vβs at the end of a 4-d culture period is shown for a range of concentrations of α-GalCer and iGb3. Data are representative of four independent experiments. ND, not determined.