Characterization of the CD4+ T cell clones specific for BLLF1, BALF4, and BZLF1
| T cell clone . | Epitope . | Restriction . | TCR-Vβ . |
|---|---|---|---|
| BLLF1-1H2 | BLLF1 AA130–144 -VYFQDVFGTMWCHHA- | HLA-DQB1*0402 | Vβ5.2 |
| BLLF1-1D6 | BLLF1 AA65–79 -FGQLTPHTKAVYQPR- | HLA-DRB1*1301 | Vβ21 |
| BZLF1-3E4 | BZLF1 AA207–221 -KSSENDRLRLLLKQM- | HLA-DQB1*0402 | Vβ21 |
| BZLF1-3H11 | BZLF1 AA174–188 -ELEIKRYKNRVASRK- | HLA-DRB1*1301 | Vβ4 |
| BALF4-B5 | BALF4 AA482–496 -AWCLEQKRQNMVLRE- | HLA-DPB1*1301 | Vβ6 |
| BALF4-A9 | BALF4 AA575–589 -DNEIFLTKKMTEVCQ- | HLA-DRB1*0801 | Vβ19 |
| T cell clone . | Epitope . | Restriction . | TCR-Vβ . |
|---|---|---|---|
| BLLF1-1H2 | BLLF1 AA130–144 -VYFQDVFGTMWCHHA- | HLA-DQB1*0402 | Vβ5.2 |
| BLLF1-1D6 | BLLF1 AA65–79 -FGQLTPHTKAVYQPR- | HLA-DRB1*1301 | Vβ21 |
| BZLF1-3E4 | BZLF1 AA207–221 -KSSENDRLRLLLKQM- | HLA-DQB1*0402 | Vβ21 |
| BZLF1-3H11 | BZLF1 AA174–188 -ELEIKRYKNRVASRK- | HLA-DRB1*1301 | Vβ4 |
| BALF4-B5 | BALF4 AA482–496 -AWCLEQKRQNMVLRE- | HLA-DPB1*1301 | Vβ6 |
| BALF4-A9 | BALF4 AA575–589 -DNEIFLTKKMTEVCQ- | HLA-DRB1*0801 | Vβ19 |
Six T cell clones recognizing two different epitopes in each of the three antigens were further characterized. The amino acid sequences and positions of the epitopes within the EBV B95.8 protein sequence are given, together with the restricting MHC molecules and the Vβ chains (TCR-Vβ) expressed by the different T cell clones.