Parameters of the Standard Multi-compartment Model
A. General . | . | . | . | . | . | . | . | . |
---|---|---|---|---|---|---|---|---|
Temperature | 16°C | |||||||
Length, z- to m-line | 2.0 μm | |||||||
Length of thin filament | 1.0 μm | |||||||
Radius of myofibril | 0.5 μm | |||||||
Resting free [Ca2+] | 0.1 μM | |||||||
Resting free [Mg2+] | 1.0 mM | |||||||
Longitudinal subdivisions | 6 | |||||||
Radial subdivisions | 3 |
A. General . | . | . | . | . | . | . | . | . |
---|---|---|---|---|---|---|---|---|
Temperature | 16°C | |||||||
Length, z- to m-line | 2.0 μm | |||||||
Length of thin filament | 1.0 μm | |||||||
Radius of myofibril | 0.5 μm | |||||||
Resting free [Ca2+] | 0.1 μM | |||||||
Resting free [Mg2+] | 1.0 mM | |||||||
Longitudinal subdivisions | 6 | |||||||
Radial subdivisions | 3 |
B. Spatial Distributions and Diffusion Constants . | . | . | . | . | ||||
---|---|---|---|---|---|---|---|---|
Species . | . | Location . | . | Diffusion constant . | . | . | . | . |
10−6 cm2 s−1 | ||||||||
Free Ca2+ | All compartments | 3 | ||||||
Troponin | Compartments within 1.0 μm of the z-line | |||||||
0 | ||||||||
Parvalbumin | All compartments | 0.15 | ||||||
ATP | All compartments | 1.4 | ||||||
Indicator dyes | All compartments | 0.20–0.99 |
B. Spatial Distributions and Diffusion Constants . | . | . | . | . | ||||
---|---|---|---|---|---|---|---|---|
Species . | . | Location . | . | Diffusion constant . | . | . | . | . |
10−6 cm2 s−1 | ||||||||
Free Ca2+ | All compartments | 3 | ||||||
Troponin | Compartments within 1.0 μm of the z-line | |||||||
0 | ||||||||
Parvalbumin | All compartments | 0.15 | ||||||
ATP | All compartments | 1.4 | ||||||
Indicator dyes | All compartments | 0.20–0.99 |
C. Bulk Concentrations and Ca2+ and Mg2+ Reaction Rates . | ||||||||
---|---|---|---|---|---|---|---|---|
Species . | . | [Total sites] . | . | k + 1 . | . | k − 1 . | . | K d . |
. | . | μM . | . | 108 M−1 s−1 . | . | s−1 . | . | μM . |
Troponin (regulatory) | 240 | 0.885 | 115 | 1.3 | ||||
Parvalbumin | 1,500 | |||||||
Ca2+ | 0.417 | 0.5 | 0.012 | |||||
Mg2+ | 0.00033 | 3.0 | 91.0 | |||||
ATP | 8,000 | 0.1364* | 30,000* | 2,200* |
C. Bulk Concentrations and Ca2+ and Mg2+ Reaction Rates . | ||||||||
---|---|---|---|---|---|---|---|---|
Species . | . | [Total sites] . | . | k + 1 . | . | k − 1 . | . | K d . |
. | . | μM . | . | 108 M−1 s−1 . | . | s−1 . | . | μM . |
Troponin (regulatory) | 240 | 0.885 | 115 | 1.3 | ||||
Parvalbumin | 1,500 | |||||||
Ca2+ | 0.417 | 0.5 | 0.012 | |||||
Mg2+ | 0.00033 | 3.0 | 91.0 | |||||
ATP | 8,000 | 0.1364* | 30,000* | 2,200* |
D. Resting Fractional Occupancies of Sites by Ca2+ and Mg2+ . | ||||||||
---|---|---|---|---|---|---|---|---|
. | . | Ca2+ . | . | Mg2+ . | . | . | . | . |
Troponin (regulatory) | 0.071 | — | ||||||
Parvalbumin | 0.410 | 0.541 | ||||||
ATP | 0.000 | 0.909* |
D. Resting Fractional Occupancies of Sites by Ca2+ and Mg2+ . | ||||||||
---|---|---|---|---|---|---|---|---|
. | . | Ca2+ . | . | Mg2+ . | . | . | . | . |
Troponin (regulatory) | 0.071 | — | ||||||
Parvalbumin | 0.410 | 0.541 | ||||||
ATP | 0.000 | 0.909* |
The table lists general information as well as typical values used for the model calculations. In B, all species are assumed to diffuse in myoplasm except for troponin, which is fixed at the location of the thin filament. In C, the site concentrations are referred to the myoplasmic water volume (cf., Baylor et al., 1983); the values of k+1, k−1, and Kd (= k−1/k+1) are for Ca2+ unless indicated otherwise. The asterisk in C and D denotes that the rates for Ca2+-ATP are effective rates in the presence of 1 mM free [Mg2+] and that a reduced set of equations involving the effective occupancy of ATP with Mg2+ was used for the majority of calculations with ATP included (see materials and methods).