Table I

Parameters of the Standard Multi-compartment Model

A. General
Temperature   16°C        
Length, z- to m-line   2.0 μm        
Length of thin filament   1.0 μm        
Radius of myofibril   0.5 μm        
Resting free [Ca2+]   0.1 μM        
Resting free [Mg2+]   1.0 mM        
Longitudinal subdivisions   6        
Radial subdivisions   3        
A. General
Temperature   16°C        
Length, z- to m-line   2.0 μm        
Length of thin filament   1.0 μm        
Radius of myofibril   0.5 μm        
Resting free [Ca2+]   0.1 μM        
Resting free [Mg2+]   1.0 mM        
Longitudinal subdivisions   6        
Radial subdivisions   3        
B. Spatial Distributions and Diffusion Constants
Species Location Diffusion constant
    10−6 cm2 s−1      
Free Ca2+   All compartments   3      
Troponin   Compartments within  1.0 μm of the z-line
 
      
   0      
Parvalbumin   All compartments   0.15      
ATP   All compartments   1.4      
Indicator dyes   All compartments   0.20–0.99      
B. Spatial Distributions and Diffusion Constants
Species Location Diffusion constant
    10−6 cm2 s−1      
Free Ca2+   All compartments   3      
Troponin   Compartments within  1.0 μm of the z-line
 
      
   0      
Parvalbumin   All compartments   0.15      
ATP   All compartments   1.4      
Indicator dyes   All compartments   0.20–0.99      
C. Bulk Concentrations and Ca2+ and Mg2+ Reaction Rates
Species [Total sites] k + 1 k − 1 K d
μM 108 M−1 s−1 s−1 μM
Troponin (regulatory)   240    0.885   115      1.3  
Parvalbumin   1,500        
 Ca2+     0.417    0.5      0.012  
 Mg2+     0.00033    3.0     91.0  
ATP   8,000   0.1364*   30,000*   2,200*  
C. Bulk Concentrations and Ca2+ and Mg2+ Reaction Rates
Species [Total sites] k + 1 k − 1 K d
μM 108 M−1 s−1 s−1 μM
Troponin (regulatory)   240    0.885   115      1.3  
Parvalbumin   1,500        
 Ca2+     0.417    0.5      0.012  
 Mg2+     0.00033    3.0     91.0  
ATP   8,000   0.1364*   30,000*   2,200*  
D. Resting Fractional Occupancies of Sites by Ca2+ and Mg2+
Ca2+ Mg2+
Troponin (regulatory)   0.071   —      
Parvalbumin   0.410   0.541      
ATP   0.000    0.909*      
D. Resting Fractional Occupancies of Sites by Ca2+ and Mg2+
Ca2+ Mg2+
Troponin (regulatory)   0.071   —      
Parvalbumin   0.410   0.541      
ATP   0.000    0.909*      

The table lists general information as well as typical values used for the model calculations. In B, all species are assumed to diffuse in myoplasm except for troponin, which is fixed at the location of the thin filament. In C, the site concentrations are referred to the myoplasmic water volume (cf., Baylor et al., 1983); the values of k+1, k−1, and Kd (= k−1/k+1) are for Ca2+ unless indicated otherwise. The asterisk in C and D denotes that the rates for Ca2+-ATP are effective rates in the presence of 1 mM free [Mg2+] and that a reduced set of equations involving the effective occupancy of ATP with Mg2+ was used for the majority of calculations with ATP included (see materials and methods).  

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