Pathogenic IFN-γ–deficient T cells induce atypical EAE
| Injected cells . | WT-IL12–treated
cells . | WT-IL23–treated
cells . | IFN-γ KO-IL12–
treated cells . | IFN-γ KO-IL23–treated
cells . |
|---|---|---|---|---|
| Incidence of disease | 5/5 | 5/5 | 14/15 | 15/15 |
| Incidence of limb dysfunction | 5/5 | 5/5 | 5/15 | 2/15 |
| Incidence of flaccid paralysis | 5/5 | 5/5 | 0/15 | 0/15 |
| Incidence of dystonia | 0/5 | 0/5 | 5/15 | 2/15 |
| Mean day of onset of clinical signs of limb dysfunction | 7.6 ± 0.6 | 20.8 ± 1.5 | 13.4 ± 4.2 | 9.0 ± 0 |
| Mean peak limb dysfunction clinical score | 2.5 ± 0 | 3.8 ± 0.5 | 1.5 ± 0.7 | 1.0 ± 0 |
| Incidence of non-classical EAE | 0/5 | 0/5 | 14/15 | 15/15 |
| Mean day of onset of clinical signs of non-classical EAE | NA | NA | 14.4 ± 4.2 | 13.1 ± 3.4 |
| Vertigo | 0/5 | 0/5 | 11/14 | 12/15 |
| Ataxia | 0/5 | 0/5 | 9/14 | 9/15 |
| Injected cells . | WT-IL12–treated
cells . | WT-IL23–treated
cells . | IFN-γ KO-IL12–
treated cells . | IFN-γ KO-IL23–treated
cells . |
|---|---|---|---|---|
| Incidence of disease | 5/5 | 5/5 | 14/15 | 15/15 |
| Incidence of limb dysfunction | 5/5 | 5/5 | 5/15 | 2/15 |
| Incidence of flaccid paralysis | 5/5 | 5/5 | 0/15 | 0/15 |
| Incidence of dystonia | 0/5 | 0/5 | 5/15 | 2/15 |
| Mean day of onset of clinical signs of limb dysfunction | 7.6 ± 0.6 | 20.8 ± 1.5 | 13.4 ± 4.2 | 9.0 ± 0 |
| Mean peak limb dysfunction clinical score | 2.5 ± 0 | 3.8 ± 0.5 | 1.5 ± 0.7 | 1.0 ± 0 |
| Incidence of non-classical EAE | 0/5 | 0/5 | 14/15 | 15/15 |
| Mean day of onset of clinical signs of non-classical EAE | NA | NA | 14.4 ± 4.2 | 13.1 ± 3.4 |
| Vertigo | 0/5 | 0/5 | 11/14 | 12/15 |
| Ataxia | 0/5 | 0/5 | 9/14 | 9/15 |
MOG35-55-specific T cell lines were generated in either C57BL/6 (WT) or IFN-γ–deficient (IFN-γ KO) mice. T cell lines were produced in the presence either of IL-12 or IL-23+TGF-β+IL-6+anti–IFN-γ as described in Materials and methods. The T cell lines were then injected i.v. Mice were examined over time for clinical signs of limb dysfunction, vertigo/disequilibrium, and ataxia. Results are representative of two separate experiments. Results are shown ± SD.