IFN-γ production in a fraction of pathogenic T cells prevents development of atypical EAE
| Transferred cells . | Incidence of nonclassical disease . | Incidence of classical EAE . | Incidence of both diseases . |
|---|---|---|---|
| WT Th1 | 0/25 | 21/25 | 0/25 |
| IFNγ−/− Th1 | 22/25 | 0/25 | 0/25 |
| WT Th1 + IFNγ−/− Th1 (1:1) | 2/25 | 24/25 | 2/25 |
| OVA Th1 + IFNγ−/− Th1 (1:1) | 17/17 | 0/17 | 0/17 |
| WT Th1 + IFNγ−/− Th1 (0.1:1) | 11/15 | 1/15 | 0/15 |
| WT Th1 + IFNγ−/− Th1 (0.2:1) | 19/30 | 16/30 | 6/30 |
| Transferred cells . | Incidence of nonclassical disease . | Incidence of classical EAE . | Incidence of both diseases . |
|---|---|---|---|
| WT Th1 | 0/25 | 21/25 | 0/25 |
| IFNγ−/− Th1 | 22/25 | 0/25 | 0/25 |
| WT Th1 + IFNγ−/− Th1 (1:1) | 2/25 | 24/25 | 2/25 |
| OVA Th1 + IFNγ−/− Th1 (1:1) | 17/17 | 0/17 | 0/17 |
| WT Th1 + IFNγ−/− Th1 (0.1:1) | 11/15 | 1/15 | 0/15 |
| WT Th1 + IFNγ−/− Th1 (0.2:1) | 19/30 | 16/30 | 6/30 |
MOG35-55-specific T cell lines were generated in either C57BL/6 (WT) or IFN-γ–deficient (IFN-γ KO) mice and polarized to a Th1 phenotype as described in Materials and methods. Ovalbumin-specific (OVA) T cell lines were also generated in C57BL/6 and polarized to a Th1 phenotype. Mice received either 5 × 106 WT, 5 × 106 IFN-γ KO, 5 × 106 WT and 5 × 106 IFN-γ KO (1:1), 5 × 106 OVA, and 5 × 106 IFN-γ KO cells (1:1), 5 × 105 WT and 5 × 106 IFN-γ KO (0.1:1), or 1 × 106 WT and 5 × 106 IFN-γ KO (0.2:1) as a single i.v. injection. Mice were examined over time for clinical signs of limb dysfunction, vertigo/disequilibrium, and ataxia. Results shown were pooled from three separate experiments. Results are shown ± SD.