Rockefeller University Press

Table IV.

Somatic mutations in J_H4-J_H5 introns flanking VDJ_H4 rearrangements from splenic and blood B cell subsets

Donors	Age  (months)	Tissue	CD20-positive  B cell  subsets	Number of sequences		Mutations
				Total	Mutated	Range	Number	Frequency/  total  sequences	Frequency/  mutated  sequences
								%	%
D3	8	spleen	IgD⁺CD27⁺	21	10  (48%)	0–6	23	0.32	0.67
			IgD⁻CD27⁺	20	19  (95%)	0–14	105	1.54	1.55
		blood	IgD⁺CD27⁺	20	12  (60%)	0–7	37	0.54	0.90
			IgD⁻CD27⁺	18	13  (72%)	0–16	103	1.67	2.32
D1	11	blood	IgD⁺CD27⁺	20	15  (75%)	0–17	79	1.16	1.54
			IgD⁻CD27⁺	20	18  (90%)	0–22	117	1.71	1.90
D2	11	blood	IgD⁺CD27⁺	18	15  (83%)	0–16	116	1.89	2.27
			IgD⁻CD27⁺	17	16  (94%)	0–21	127	2.19	2.32
D4	22	spleen	IgD⁺CD27⁺	25	12  (48%)	0–14	40	0.51	1.09
			IgD⁻CD27⁺	14	13  (93%)	0–23	91	1.90	2.05

Donors	Age  (months)	Tissue	CD20-positive  B cell  subsets	Number of sequences		Mutations
				Total	Mutated	Range	Number	Frequency/  total  sequences	Frequency/  mutated  sequences
								%	%
D3	8	spleen	IgD⁺CD27⁺	21	10  (48%)	0–6	23	0.32	0.67
			IgD⁻CD27⁺	20	19  (95%)	0–14	105	1.54	1.55
		blood	IgD⁺CD27⁺	20	12  (60%)	0–7	37	0.54	0.90
			IgD⁻CD27⁺	18	13  (72%)	0–16	103	1.67	2.32
D1	11	blood	IgD⁺CD27⁺	20	15  (75%)	0–17	79	1.16	1.54
			IgD⁻CD27⁺	20	18  (90%)	0–22	117	1.71	1.90
D2	11	blood	IgD⁺CD27⁺	18	15  (83%)	0–16	116	1.89	2.27
			IgD⁻CD27⁺	17	16  (94%)	0–21	127	2.19	2.32
D4	22	spleen	IgD⁺CD27⁺	25	12  (48%)	0–14	40	0.51	1.09
			IgD⁻CD27⁺	14	13  (93%)	0–23	91	1.90	2.05