Mice with a T reg cell–specific Dicer deficiency exhibit sharply increased CD4 T cell cytokine production
. | Foxp3creDicerwt/wt . | Foxp3KO . | CD4-Cre Dicerfl/fl . | Foxp3creDicerfl/fl . |
|---|---|---|---|---|
| IFN-γ | 1.99 ± 2.47 | 4.64 ± 0.31 | 1.36 ± 1.67 | 10.39 ± 5.93 |
| TNF-α | 26.53 ± 4.26 | 10.61 ± 0.46 | 32.18 ± 4.13 | 23.94 ± 4.44 |
| IL-2 | 1.01 ± 0.6 | 3.9 ± 0.5 | 0.48 ± 0.17 | 7.77 ± 1.81 |
| IL-4 | 0.38 ± 0.28 | 2.65 ± 0.69 | 2.31 ± 1.86 | 3.85 ± 1.7 |
| IL-5 | 0.9 ± 0.48 | 2.48 ± 1.08 | 0.75 ± 0.19 | 3.54 ± 1.06 |
| IL-17 | 0.49 ± 0.25 | 1.2 ± 0.03 | 0.8 ± 0.64 | 1.81 ± 0.42 |
. | Foxp3creDicerwt/wt . | Foxp3KO . | CD4-Cre Dicerfl/fl . | Foxp3creDicerfl/fl . |
|---|---|---|---|---|
| IFN-γ | 1.99 ± 2.47 | 4.64 ± 0.31 | 1.36 ± 1.67 | 10.39 ± 5.93 |
| TNF-α | 26.53 ± 4.26 | 10.61 ± 0.46 | 32.18 ± 4.13 | 23.94 ± 4.44 |
| IL-2 | 1.01 ± 0.6 | 3.9 ± 0.5 | 0.48 ± 0.17 | 7.77 ± 1.81 |
| IL-4 | 0.38 ± 0.28 | 2.65 ± 0.69 | 2.31 ± 1.86 | 3.85 ± 1.7 |
| IL-5 | 0.9 ± 0.48 | 2.48 ± 1.08 | 0.75 ± 0.19 | 3.54 ± 1.06 |
| IL-17 | 0.49 ± 0.25 | 1.2 ± 0.03 | 0.8 ± 0.64 | 1.81 ± 0.42 |
Splenocytes isolated from mice of the indicated genotypes were stimulated in vitro with 1 μg/ml CD3 and 1 μg/ml CD28 antibodies for 5 h and assessed for cytokine production by intracellular staining. Values shown represent the mean and standard deviation of the percentage of CD4+Foxp3− cells producing the corresponding cytokines (n = 4–7).