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Table 1.

Histomorphometry analysis of Dcamkl1−/− mice

Parameter WT (n = 6) Dcamkl1−/− (n ≥ 5) P-value 
BV/TV (%) 6.13 ± 0.74 10.39 ± 1.32a 0.018 
Tb.Th (µm) 28.22 ± 1.01 34.23 ± 1.42b 0.006 
Tb.N (/mm) 2.18 ± 0.25 3.01 ± 0.29 0.055 
Tb.Sp (µm) 464 ± 56 316 ± 39 0.055 
MS/BS (%) 36.68 ± 1.80 42.16 ± 1.38a 0.045 
MAR (µm/d) 1.81 ± 0.05 2.16 ± 0.14a 0.033 
BFR/BS (µm3/µm2/yr) 242 ± 13 331 ± 20b 0.004 
BFR/BV (%/yr) 1,711 ± 36 2,016 ± 142a 0.048 
BFR/TV (%/yr) 105 ± 12 184 ± 12b 0.001 
Ob.S/BS (%) 10.19 ± 2.14 15.55 ± 1.27 0.057 
N.Ob/T.Ar (/mm233.96 ± 5.19 81.52 ± 14.18a 0.010 
N.Ob/BS (/mm) 8.68 ± 1.62 13.26 ± 1.11a 0.042 
OS/BS (%) 5.56 ± 1.01 5.88 ± 0.94 0.824 
O.Th (µm) 2.49 ± 0.33 2.40 ± 0.29 0.854 
Oc.S/BS (%) 0.84 ± 0.21 0.57 ± 0.14 0.303 
N.Oc/T.Ar (/mm21.74 ± 0.59 1.54 ± 0.38 0.783 
N.Oc/BS (/mm) 0.37 ± 0.08 0.24 ± 0.06 0.205 
Parameter WT (n = 6) Dcamkl1−/− (n ≥ 5) P-value 
BV/TV (%) 6.13 ± 0.74 10.39 ± 1.32a 0.018 
Tb.Th (µm) 28.22 ± 1.01 34.23 ± 1.42b 0.006 
Tb.N (/mm) 2.18 ± 0.25 3.01 ± 0.29 0.055 
Tb.Sp (µm) 464 ± 56 316 ± 39 0.055 
MS/BS (%) 36.68 ± 1.80 42.16 ± 1.38a 0.045 
MAR (µm/d) 1.81 ± 0.05 2.16 ± 0.14a 0.033 
BFR/BS (µm3/µm2/yr) 242 ± 13 331 ± 20b 0.004 
BFR/BV (%/yr) 1,711 ± 36 2,016 ± 142a 0.048 
BFR/TV (%/yr) 105 ± 12 184 ± 12b 0.001 
Ob.S/BS (%) 10.19 ± 2.14 15.55 ± 1.27 0.057 
N.Ob/T.Ar (/mm233.96 ± 5.19 81.52 ± 14.18a 0.010 
N.Ob/BS (/mm) 8.68 ± 1.62 13.26 ± 1.11a 0.042 
OS/BS (%) 5.56 ± 1.01 5.88 ± 0.94 0.824 
O.Th (µm) 2.49 ± 0.33 2.40 ± 0.29 0.854 
Oc.S/BS (%) 0.84 ± 0.21 0.57 ± 0.14 0.303 
N.Oc/T.Ar (/mm21.74 ± 0.59 1.54 ± 0.38 0.783 
N.Oc/BS (/mm) 0.37 ± 0.08 0.24 ± 0.06 0.205 

Tb.Sp, trabecular separation; N.Ob/T.Ar, osteoblast number/tissue area ratio; N.Ob/BS, osteoblast number/bone surface; N.Oc/T.Ar, osteoclast number/tissue area ratio; N.Oc/BS, osteoclast number/bone surface. 9-wk-old male Dcamkl1−/− mice and WT control mice were injected with calcein. 5 d later, mice were injected with calcein again. Mice were sacrificed 3 d after the second injection, and tibias were processed for quantitative histomorphometry. Histomorphometric analysis at proximal tibiae revealed that Dcamkl1−/− mice had significantly higher cancellous bone volume and thicker trabeculae than WT controls.

a

P < 0.05 compared with WT, unpaired Student’s t test.

b

P < 0.01 compared with WT, unpaired Student’s t test.

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