Skip to Main Content
TABLE I

Effect of CaM and CaM1234 Overexpression on NaV1.4, NaV1.4-EYFP and NaV1.41740-EYFP

Channel/Mutant
V1/2 steady state inactivation
Decay time constant τh
V1/2 activation
Current density
 mV ms at −20 mV mV pA/pF 
NaV1.4-EYFP + β1 −65.2 ± 0.4 (n = 5) 0.69 ± 0.04 −32.2 ± 0.1 −426.4 ± 93.3 
NaV1.4-EYFP + ECFP-CaM + β1 −71 ± 0.2 (n = 5) 0.77 ± 0.05 −32.6 ± 0.2 −458.0 ± 54.0 
NaV1.4-EYFP + ECFP-CaM1234 + β1 −64.2 ± 0.1 (n = 5) 0.66 ± 0.02 −32.9 ± 0.1 −269.6 ± 55.5 
NaV1.4 + β1 −65.08 ± 0.1 (n = 6) 0.61 ± 0.07 −32.1 ± 0.3 −453.2 ± 71.5 
NaV1.4 + ECFP-CaM + β1 −69.3 ± 0.1 (n = 7) 0.63 ± 0.02 −30.6 ± 0.2 −309.4 ± 112.0 
NaV1.4 + ECFP-CaM1234 + β1 −64.3 ± 0.3 (n = 7) 0.66 ± 0.07 −31.2 ± 0.1 −324.1 ± 80.9 
NaV1.4IQ/AA + β1 −79.4 ± 0.2 (n = 6) 1.3 ± 0.2 −40.9 ± 0.3 −307.8 ± 89.9 
NaV1.4IQ/AA + ECFP-CaM + β1 −80.0 ± 0.4 (n = 6) 1.25 ± 0.15 −40.0 ± 0.1 −242.5 ± 60.5 
NaV1.4IQ/AA + ECFP-CaM1234 + β1 −80.7 ± 0.3 (n =5) 1.12 ± 0.15 −41.0 ± 0.3 −254.2 ± 73.1 
NaV1.41740 + β1 −62.7 ± 0.1 (n = 6) 0.69 ± 0.09 −30.1 ± 0.2 −437.8 ± 116.0 
NaV1.41740 + ECFP-CaM + β1 −68.0 ± 0.1 (n = 8) 0.62 ± 0.02 −29.5 ± 0.1 −294.0 ± 74.7 
NaV1.41740 + ECFP-CaM1234 + β1 −64.0 ± 0.04 (n = 5) 0.79 ± 0.01 −31.1 ± 0.2 −295.0 ± 60.9 
NaV1.41740-G4-CaM + β1 −65.8 ± 0.1 (n = 8) 0.69 ± 0.09 −30.8 ± 0.3 −372.9 ± 67.4 
NaV1.41740-G4-CaM + ECFP-CaM + β1 −71.4 ± 0.2 (n = 9) 0.55 ± 0.3 −30.7 ± 0.1 −373.0 ± 77.5 
NaV1.41740-G4-CaM + ECFP-CaM1234 + β1 −63.6 ± 0.2 (n = 5) 0.52 ± 0.1 −32.1 ± 0.2 −413.5 ± 118.9 
NaV1.41740-G14-CaM + β1 −68.4 ± 0.2 (n = 9) 0.60 ± 0.1 −31.9 ± 0.3 −201.4 ± 63.2 
NaV1.41740-G14-CaM + ECFP-CaM + β1 −67.7 ± 0.2 (n = 9) 0.51 ± 0.06 −30.2 ± 0.3 −391.5 ± 121.1 
NaV1.41740-G14-CaM + ECFP-CaM1234 + β1 −66.7 + 0.1 (n = 9) 0.53 ± 0.06 −32.0 ± 0.1 −439.5 ± 141.9  
Channel/Mutant
V1/2 steady state inactivation
Decay time constant τh
V1/2 activation
Current density
 mV ms at −20 mV mV pA/pF 
NaV1.4-EYFP + β1 −65.2 ± 0.4 (n = 5) 0.69 ± 0.04 −32.2 ± 0.1 −426.4 ± 93.3 
NaV1.4-EYFP + ECFP-CaM + β1 −71 ± 0.2 (n = 5) 0.77 ± 0.05 −32.6 ± 0.2 −458.0 ± 54.0 
NaV1.4-EYFP + ECFP-CaM1234 + β1 −64.2 ± 0.1 (n = 5) 0.66 ± 0.02 −32.9 ± 0.1 −269.6 ± 55.5 
NaV1.4 + β1 −65.08 ± 0.1 (n = 6) 0.61 ± 0.07 −32.1 ± 0.3 −453.2 ± 71.5 
NaV1.4 + ECFP-CaM + β1 −69.3 ± 0.1 (n = 7) 0.63 ± 0.02 −30.6 ± 0.2 −309.4 ± 112.0 
NaV1.4 + ECFP-CaM1234 + β1 −64.3 ± 0.3 (n = 7) 0.66 ± 0.07 −31.2 ± 0.1 −324.1 ± 80.9 
NaV1.4IQ/AA + β1 −79.4 ± 0.2 (n = 6) 1.3 ± 0.2 −40.9 ± 0.3 −307.8 ± 89.9 
NaV1.4IQ/AA + ECFP-CaM + β1 −80.0 ± 0.4 (n = 6) 1.25 ± 0.15 −40.0 ± 0.1 −242.5 ± 60.5 
NaV1.4IQ/AA + ECFP-CaM1234 + β1 −80.7 ± 0.3 (n =5) 1.12 ± 0.15 −41.0 ± 0.3 −254.2 ± 73.1 
NaV1.41740 + β1 −62.7 ± 0.1 (n = 6) 0.69 ± 0.09 −30.1 ± 0.2 −437.8 ± 116.0 
NaV1.41740 + ECFP-CaM + β1 −68.0 ± 0.1 (n = 8) 0.62 ± 0.02 −29.5 ± 0.1 −294.0 ± 74.7 
NaV1.41740 + ECFP-CaM1234 + β1 −64.0 ± 0.04 (n = 5) 0.79 ± 0.01 −31.1 ± 0.2 −295.0 ± 60.9 
NaV1.41740-G4-CaM + β1 −65.8 ± 0.1 (n = 8) 0.69 ± 0.09 −30.8 ± 0.3 −372.9 ± 67.4 
NaV1.41740-G4-CaM + ECFP-CaM + β1 −71.4 ± 0.2 (n = 9) 0.55 ± 0.3 −30.7 ± 0.1 −373.0 ± 77.5 
NaV1.41740-G4-CaM + ECFP-CaM1234 + β1 −63.6 ± 0.2 (n = 5) 0.52 ± 0.1 −32.1 ± 0.2 −413.5 ± 118.9 
NaV1.41740-G14-CaM + β1 −68.4 ± 0.2 (n = 9) 0.60 ± 0.1 −31.9 ± 0.3 −201.4 ± 63.2 
NaV1.41740-G14-CaM + ECFP-CaM + β1 −67.7 ± 0.2 (n = 9) 0.51 ± 0.06 −30.2 ± 0.3 −391.5 ± 121.1 
NaV1.41740-G14-CaM + ECFP-CaM1234 + β1 −66.7 + 0.1 (n = 9) 0.53 ± 0.06 −32.0 ± 0.1 −439.5 ± 141.9  
Close Modal

or Create an Account

Close Modal
Close Modal