Rockefeller University Press

TABLE I

Parameters Derived from the Variations in Rates of Ryanoid Association with, and Dissociation from, Individual RyR2 Channels and Binding to Populations of Channels in Intact SR Vesicles

	10-O-succinoylryanodol  (formal charge −1)	Ryanodol  (formal charge 0)	21-amino-9a-hydroxyryanodine  (formal charge +1)
Slope (z_onF/RT)	0.021 ± 0.003 (r = 0.75)	0.041 ± 0.003 (r = 1)	0.051 ± 0.002 (r = 1)
z_on	0.54	1.03	1.29
Slope (−z_offF/RT)	−0.028 ± 0.005 (r = 0.75)	−0.019 ± 0.002 (r = 1)	−0.034 ± 0.002 (r = 1)
z_off	0.70	0.48	0.87
z_total	1.24	1.51	2.16
k_on at 0 mV (μM⁻¹s⁻¹)	0.002	0.035	0.365
k_off at 0 mV (s⁻¹)	0.082	0.095	0.990
K_d at 0 mV (μM)	35.620	2.810	2.790
K_d (binding, μM)	19.5 ± 4.0^{^a}	7.0 ± 2.0,^{^a} 5.8 ± 0.9^{^b}	3.7 ± 0.6^{^b}

	10-O-succinoylryanodol  (formal charge −1)	Ryanodol  (formal charge 0)	21-amino-9a-hydroxyryanodine  (formal charge +1)
Slope (z_onF/RT)	0.021 ± 0.003 (r = 0.75)	0.041 ± 0.003 (r = 1)	0.051 ± 0.002 (r = 1)
z_on	0.54	1.03	1.29
Slope (−z_offF/RT)	−0.028 ± 0.005 (r = 0.75)	−0.019 ± 0.002 (r = 1)	−0.034 ± 0.002 (r = 1)
z_off	0.70	0.48	0.87
z_total	1.24	1.51	2.16
k_on at 0 mV (μM⁻¹s⁻¹)	0.002	0.035	0.365
k_off at 0 mV (s⁻¹)	0.082	0.095	0.990
K_d at 0 mV (μM)	35.620	2.810	2.790
K_d (binding, μM)	19.5 ± 4.0^{^a}	7.0 ± 2.0,^{^a} 5.8 ± 0.9^{^b}	3.7 ± 0.6^{^b}

^a

Sheep cardiac SR.

^b

Rabbit skeletal SR.