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TABLE II

Substituted Cysteine Modification Rates in ConditionsFavoring Fast or Slow Inactivation


Mutant

Fast inactivated

n

Slow inactivated

n

 
M−1s−1
 

 
M−1s−1
 

 
Y401C 350 ± 35 270 ± 63 
E403C 17,000 ± 4,300 16,000 ± 2700 
E758C 1,600 ± 380 2,800 ± 490 
M1240C 180 ± 17 220 ± 8.8 
G1530C 49 ± 11 56 ± 9.8 
D1532C
 
7,700 ± 2,600
 
4
 
8,700 ± 720
 
5
 

Mutant

Fast inactivated

n

Slow inactivated

n

 
M−1s−1
 

 
M−1s−1
 

 
Y401C 350 ± 35 270 ± 63 
E403C 17,000 ± 4,300 16,000 ± 2700 
E758C 1,600 ± 380 2,800 ± 490 
M1240C 180 ± 17 220 ± 8.8 
G1530C 49 ± 11 56 ± 9.8 
D1532C
 
7,700 ± 2,600
 
4
 
8,700 ± 720
 
5
 
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