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TABLE II

Kinetic Parameters of Barium Binding to KCNQ1 Channels in High External K+ Solutions



Wash-in

Wash-in

Washout

Washout
Fast τSlow τFast τSlow τ

 
ms
 
ms
 
ms
 
ms
 
0 mV 319 ± 37 6,472 ± 806 511 ± 49 11,810 ± 1,879 
10 mV 368 ± 58 7,615 ± 1,269 576 ± 88 13,774 ± 2,038 
20 mV 532 ± 149 8,173 ± 1,567 713 ± 145 11,958 ± 1,715 
30 mV
 
781 ± 276
 
12,047 ± 1,703
 
1,013 ± 437
 
13,134 ± 1,769
 


Wash-in

Wash-in

Washout

Washout
Fast τSlow τFast τSlow τ

 
ms
 
ms
 
ms
 
ms
 
0 mV 319 ± 37 6,472 ± 806 511 ± 49 11,810 ± 1,879 
10 mV 368 ± 58 7,615 ± 1,269 576 ± 88 13,774 ± 2,038 
20 mV 532 ± 149 8,173 ± 1,567 713 ± 145 11,958 ± 1,715 
30 mV
 
781 ± 276
 
12,047 ± 1,703
 
1,013 ± 437
 
13,134 ± 1,769
 

On- and off-binding kinetics of extracellular barium were measured in high external K+ solutions (50 mM) by applying 10 mM Ba2+ to KCNQ1 channels previously opened at the indicated potentials. Wash-in and washout kinetics were fitted by a double exponential function and the fast and slow time constants are indicated (mean ± SEM, n = 5).

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