Energy and Area Differences between the Closed (C), Substates S0.13 and S0.22, and Fully Open State (O) Determined from the Slopes of their Occupancy Dependencies on Tension for WT MscL and the Three Mutants
. | WTa (n = 10) . | . | V23T (n = 9) . | . | V23D (n = 6) . | . | G22N (n = 3) . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substate | ΔE, kT | ΔA, nm2 | ΔE, kT | ΔA, nm2 | ΔE, kT | ΔA, nm2 | ΔE, kT | ΔA, nm2 | ||||
| C | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | ||||
| S0.13 | 45.6 | 14.8 | 27 ± 8 | 11 ± 3 | 12 ± 5 | 10 ± 2.5 | 0.9 ± 2.0 | 3.0 ± 0.2 | ||||
| S0..22 | 47.0 | 15.7 | 31 ± 10 | 12.5 ± 4 | 16.5 ± 5 | 11.0 ± 3 | 3.7 ± 1.4 | 3.9 ± 0.6 | ||||
| O | 52 ± 13.0 | 20 ± 5 | 41 ± 14 | 18 ± 6 | 26 ± 4 | 15 ± 2 | 13 ± 3 | 12 ± 1.7 | ||||
. | WTa (n = 10) . | . | V23T (n = 9) . | . | V23D (n = 6) . | . | G22N (n = 3) . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substate | ΔE, kT | ΔA, nm2 | ΔE, kT | ΔA, nm2 | ΔE, kT | ΔA, nm2 | ΔE, kT | ΔA, nm2 | ||||
| C | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | ||||
| S0.13 | 45.6 | 14.8 | 27 ± 8 | 11 ± 3 | 12 ± 5 | 10 ± 2.5 | 0.9 ± 2.0 | 3.0 ± 0.2 | ||||
| S0..22 | 47.0 | 15.7 | 31 ± 10 | 12.5 ± 4 | 16.5 ± 5 | 11.0 ± 3 | 3.7 ± 1.4 | 3.9 ± 0.6 | ||||
| O | 52 ± 13.0 | 20 ± 5 | 41 ± 14 | 18 ± 6 | 26 ± 4 | 15 ± 2 | 13 ± 3 | 12 ± 1.7 | ||||
The standard deviations are not shown because the ΔE and ΔA parameters for WT were determined from one unified dataset based on 10 independent traces.