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Table I.

FRET efficiency values are indicated for liposomes imaged before and after the addition of LPC

Mutant FRET pre-LPC FRET post-LPC Δ Diameter FRET Δ Diameter simulation 
   Å Å 
S2Ca 0.22 ± 0.02 (24) 0.11 ± 0.02 (30) 22 ± 6 18.7 
K5Ca 0.23 ± 0.01 (25) 0.14 ± 0.02 (25) 16 ± 4 17.5 
M12C 0.45 ± 0.01 (30) 0.46 ± 0.01 (36) −1 ± 1 17.4 
G14C 0.38 ± 0.02 (11) 0.40 ± 0.02 (12) −2 ± 3 22.9 
I25C 0.16 ± 0.02 (20) 0.15 ± 0.02 (34) 2 ± 6 24.6 
A27Ca 0.23 ± 0.01 (23) 0.09 ± 0.01 (19) 29 ± 4 25.9 
V33Ca 0.19 ± 0.01 (21) 0.12 ± 0.01 (26) 14 ± 3 17.4 
M42Ca 0.61 ± 0.01 (8) 0.44 ± 0.01 (9) 16 ± 2 15.1 
K55C 0.06 ± 0.02 (18) 0.03 ± 0.01 (16) 23 ± 15 20.1 
Q65C 0.09 ± 0.03 (25) 0.05 ± 0.03 (17) 18 ± 18 14.3 
M73C 0.17 ± 0.02 (39) 0.17 ± 0.02 (35) 0 ± 5 14.1 
I86Ca 0.21 ± 0.02 (46) 0.14 ± 0.02 (34) 12 ± 5 17.5 
M94Ca 0.14 ± 0.01 (51) 0.08 ± 0.01 (65) 16 ± 5 20.1 
V120Ca 0.78 ± 0.01 (28) 0.66 ± 0.02 (49) 14 ± 2 12.7 
Mutant FRET pre-LPC FRET post-LPC Δ Diameter FRET Δ Diameter simulation 
   Å Å 
S2Ca 0.22 ± 0.02 (24) 0.11 ± 0.02 (30) 22 ± 6 18.7 
K5Ca 0.23 ± 0.01 (25) 0.14 ± 0.02 (25) 16 ± 4 17.5 
M12C 0.45 ± 0.01 (30) 0.46 ± 0.01 (36) −1 ± 1 17.4 
G14C 0.38 ± 0.02 (11) 0.40 ± 0.02 (12) −2 ± 3 22.9 
I25C 0.16 ± 0.02 (20) 0.15 ± 0.02 (34) 2 ± 6 24.6 
A27Ca 0.23 ± 0.01 (23) 0.09 ± 0.01 (19) 29 ± 4 25.9 
V33Ca 0.19 ± 0.01 (21) 0.12 ± 0.01 (26) 14 ± 3 17.4 
M42Ca 0.61 ± 0.01 (8) 0.44 ± 0.01 (9) 16 ± 2 15.1 
K55C 0.06 ± 0.02 (18) 0.03 ± 0.01 (16) 23 ± 15 20.1 
Q65C 0.09 ± 0.03 (25) 0.05 ± 0.03 (17) 18 ± 18 14.3 
M73C 0.17 ± 0.02 (39) 0.17 ± 0.02 (35) 0 ± 5 14.1 
I86Ca 0.21 ± 0.02 (46) 0.14 ± 0.02 (34) 12 ± 5 17.5 
M94Ca 0.14 ± 0.01 (51) 0.08 ± 0.01 (65) 16 ± 5 20.1 
V120Ca 0.78 ± 0.01 (28) 0.66 ± 0.02 (49) 14 ± 2 12.7 

The number of liposomes used in the analysis is indicated in brackets in each case. The corresponding change in diameter at the location of the fluorophores calculated from the FRET data and that seen in the simulations (60 mN/m) are also indicated.

a

Residues used as restraints in the MD simulations.

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