Depletion of Cellular Cholesterol Allows Expression of HA 2A520 to Become More Apical but Has No Effect on the Distribution of Wild-Type HA or HA+8 D549H, a Basolaterally Expressed HA Mutant*
| Protein . | . | Cholesterol depletion . | . | Percent apical . | . | Trials . |
|---|---|---|---|---|---|---|
| Wild-type HA | − | 70 ± 5 | 3 | |||
| Wild-type HA | + | 69 ± 6 | 5 | |||
| HA 2A520 | − | 20 ± 7 | 3 | |||
| HA 2A520 | + | 49 ± 12 | 4 | |||
| HA+8 D549H | − | 14 ± 4 | 3 | |||
| HA+8 D549H | + | 15 ± 6 | 4 |
| Protein . | . | Cholesterol depletion . | . | Percent apical . | . | Trials . |
|---|---|---|---|---|---|---|
| Wild-type HA | − | 70 ± 5 | 3 | |||
| Wild-type HA | + | 69 ± 6 | 5 | |||
| HA 2A520 | − | 20 ± 7 | 3 | |||
| HA 2A520 | + | 49 ± 12 | 4 | |||
| HA+8 D549H | − | 14 ± 4 | 3 | |||
| HA+8 D549H | + | 15 ± 6 | 4 |
Cholesterol was depleted from MDCK cell monolayers expressing each of the HA proteins by treatment overnight in cholesterol-free medium containing 50 μM of compactin according to published methods (Hua et al., 1996). Distribution of HAs at the cell surface was measured as described for Table II. Values are presented ± SD.
Lin et al., 1997.