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Table I

Cytosolic pH Is Slightly Acidified in ldlF Cells

pH/pKa34°C40°C
wt  7.44/7.21  7.43/7.15 
ldlF  7.18/7.30  7.19/7.35 
ldlF[LDLF]  7.30/7.32  7.41/7.24 
pH/pKa34°C40°C
wt  7.44/7.21  7.43/7.15 
ldlF  7.18/7.30  7.19/7.35 
ldlF[LDLF]  7.30/7.32  7.41/7.24 

Acidification of cytosolic pH can cause the redistribution of endocytic structures. We therefore measured cytosolic pH using the fluorescent probe BCECF in each of the cell types after incubation for 8 h at either the permissive (34°C) or nonpermissive (40°C) temperatures. For each condition, a standard curve was generated to calculate both the pKa of the dye and the intracellular pH. The mutant cells were only slightly acidified. Values are from one experiment wherein a standard curve was prepared for each condition. Similar results were obtained for wt cells and ldlF cells at 40°C in several other experiments.  

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