AnnexinV Staining
| . | . | % negative (peak mean) . | . | % positive (peak mean) . |
|---|---|---|---|---|
| OPN | ||||
| 24 h | 90.6 (62.59) | 9.4 (106.2) | ||
| 48 h | 88.9 (92.36) | 11.1 (186.97) | ||
| PDL | ||||
| 24 h | 69.9 (76.11) | 30.4 (145.01) | ||
| 48 h | 50.2 (105.52) | 49.08 (192.25) |
| . | . | % negative (peak mean) . | . | % positive (peak mean) . |
|---|---|---|---|---|
| OPN | ||||
| 24 h | 90.6 (62.59) | 9.4 (106.2) | ||
| 48 h | 88.9 (92.36) | 11.1 (186.97) | ||
| PDL | ||||
| 24 h | 69.9 (76.11) | 30.4 (145.01) | ||
| 48 h | 50.2 (105.52) | 49.08 (192.25) |
Cells were plated on osteopontin (OPN) or polylysine (PDL). 24 and 48 h after plating, cells were harvested and stained with AnnexinV-FITC and analyzed by flow cytometry. Negative and positive percentage values at the two timepoints are indicated. In addition, the peak mean (in parentheses) is also indicated.